Flow cytometry: Unravelling the real antimicrobial and antibiofilm efficacy of natural bioactive compounds.

Autor: Poscente V; Department for Sustainability, Biotechnologies and Agroindustry Division, ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, 00123 Rome, Italy; Department of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, Italy., Di Gregorio L; Department for Sustainability, Biotechnologies and Agroindustry Division, ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, 00123 Rome, Italy. Electronic address: luciana.digregorio@enea.it., Costanzo M; Department for Sustainability, Biotechnologies and Agroindustry Division, ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, 00123 Rome, Italy., Bernini R; Department of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, Italy., Bevivino A; Department for Sustainability, Biotechnologies and Agroindustry Division, ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, 00123 Rome, Italy.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2024 Jul; Vol. 222, pp. 106956. Date of Electronic Publication: 2024 May 15.
DOI: 10.1016/j.mimet.2024.106956
Abstrakt: Flow cytometry (FCM) provides unique information on bacterial viability and physiology, allowing a real-time early warning antimicrobial and antibiofilm monitoring system for preventing the spread risk of foodborne disease. The present work used a combined culture-based and FCM approach to assess the in vitro efficacy of essential oils (EOs) from condiment plants commonly used in Mediterranean Europe (i.e., thyme EO, oregano EO, basil EO, and lemon EO) against planktonic and sessile cells of food-pathogenic Listeria monocytogenes 56 LY, and contaminant and alterative species Escherichia coli ATCC 25922 and Pseudomonas fluorescens ATCC 13525. Evaluation of the bacterial response to the increasing concentrations of natural compounds posed FCM as a crucial technique for the quantification of the live/dead, and viable but non-culturable (VBNC) cells when antimicrobial agents exert no real bactericidal action. Furthermore, the FCM results displayed higher numbers of viable bacteria expressed as Active Fluorescent Units (AFUs) with a greater level of repeatability compared with outcomes of the plate-count method. Overall, accurate counting of viable microbial cells is a critically important parameter in food microbiology, and flow cytometry provides an innovative approach with high-throughput potential for applications in the food industry as "flow microbiology".
Competing Interests: Declaration of competing interest Annamaria Bevivino reports financial support was provided by European Commission—NextGenerationEU programme Project SUS-MIRRI.IT “Strengthening the MIRRI Italian Research Infrastructure for Sustainable Bioscience and Bioeconomy,” Project code IR0000005, CUP D13C22001390001. Luciana Di Gregorio reports financial support was provided by the European Commission—NextGenerationEU programme Project ON Foods—Research and innovation network on food and nutrition Sustainability, Safety and Security—Working ON Foods, Project code PE00000003, CUP I83C22001790001; and European Commission—NextGenerationEU programme Project SUS-MIRRI.IT “Strengthening the MIRRI Italian Research Infrastructure for Sustainable Bioscience and Bioeconomy,” Project code IR0000005, CUP D13C22001390001. Manuela Costanzo reports financial support was provided by the European Commission—NextGenerationEU programme Project SUS-MIRRI.IT “Strengthening the MIRRI Italian Research Infrastructure for Sustainable Bioscience and Bioeconomy,” Project code IR0000005, CUP D13C22001390001. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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