Bioengineering methods for vascularizing organoids.

Autor:	Nwokoye PN; Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Abilez OJ; Department of Cardiothoracic Surgery, Stanford University, Stanford, CA 94305, USA; Division of Pediatric CT Surgery, Stanford University, Stanford, CA 94305, USA; Cardiovascular Institute, Stanford University, Stanford, CA 94305, USA; Maternal and Child Health Research Institute, Stanford University, Stanford, CA 94305, USA; Bio-X Program, Stanford University, Stanford, CA 94305, USA. Electronic address: ojabilez@stanford.edu.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2024 Jun 17; Vol. 4 (6), pp. 100779. Date of Electronic Publication: 2024 May 16.
DOI:	10.1016/j.crmeth.2024.100779
Abstrakt:	Organoids, self-organizing three-dimensional (3D) structures derived from stem cells, offer unique advantages for studying organ development, modeling diseases, and screening potential therapeutics. However, their translational potential and ability to mimic complex in vivo functions are often hindered by the lack of an integrated vascular network. To address this critical limitation, bioengineering strategies are rapidly advancing to enable efficient vascularization of organoids. These methods encompass co-culturing organoids with various vascular cell types, co-culturing lineage-specific organoids with vascular organoids, co-differentiating stem cells into organ-specific and vascular lineages, using organoid-on-a-chip technology to integrate perfusable vasculature within organoids, and using 3D bioprinting to also create perfusable organoids. This review explores the field of organoid vascularization, examining the biological principles that inform bioengineering approaches. Additionally, this review envisions how the converging disciplines of stem cell biology, biomaterials, and advanced fabrication technologies will propel the creation of increasingly sophisticated organoid models, ultimately accelerating biomedical discoveries and innovations. Competing Interests: Declaration of interests Stanford University has filed a patent application that covers the generation of vascularized organoids (US patent application numbers 63/314,958 and US2023/013997). O.J.A. is a co-founder of Bullseye Biotechnologies and a consultant for Rosebud Biosciences and CytoHub. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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