Near Infrared Autofluorescence Lifetime Imaging of Human Retinal Pigment Epithelium Using Adaptive Optics Scanning Light Ophthalmoscopy.

Autor:	Kunala K; Center for Visual Science, University of Rochester, Rochester, New York, United States.; Byers Eye Institute, Stanford University, Palo Alto, California, United States., Tang JAH; Center for Visual Science, University of Rochester, Rochester, New York, United States.; The Institute of Optics, University of Rochester, Rochester, New York, United States., Bowles Johnson KE; Center for Visual Science, University of Rochester, Rochester, New York, United States.; Flaum Eye Institute, University of Rochester, Rochester, New York, United States.; School of Optometry, Indiana University, Bloomington, Indiana, United States., Huynh KT; Center for Visual Science, University of Rochester, Rochester, New York, United States.; Department of Biomedical Engineering, University of Rochester, Rochester, New York, United States.; Herbert Wertheim School of Optometry & Vision Science, University of California, Berkeley, Berkeley, California, United States., Parkins K; Center for Visual Science, University of Rochester, Rochester, New York, United States., Kim HJ; College of Pharmacy, Keimyung University, Dalseo-gu, Daegu, South Korea.; Department of Ophthalmology, Columbia University Medical Center, New York, New York, United States., Yang Q; Center for Visual Science, University of Rochester, Rochester, New York, United States., Sparrow JR; Department of Ophthalmology, Columbia University Medical Center, New York, New York, United States., Hunter JJ; Center for Visual Science, University of Rochester, Rochester, New York, United States.; Flaum Eye Institute, University of Rochester, Rochester, New York, United States.; School of Optometry and Vision Science, University of Waterloo, Waterloo, Ontario, Canada.
Jazyk:	angličtina
Zdroj:	Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2024 May 01; Vol. 65 (5), pp. 27.
DOI:	10.1167/iovs.65.5.27
Abstrakt:	Purpose: To demonstrate the first near-infrared adaptive optics fluorescence lifetime imaging ophthalmoscopy (NIR-AOFLIO) measurements in vivo of the human retinal pigment epithelial (RPE) cellular mosaic and to visualize lifetime changes at different retinal eccentricities. Methods: NIR reflectance and autofluorescence were captured using a custom adaptive optics scanning light ophthalmoscope in 10 healthy subjects (23-64 years old) at seven eccentricities and in two eyes with retinal abnormalities. Repeatability was assessed across two visits up to 8 weeks apart. Endogenous retinal fluorophores and hydrophobic whole retinal extracts of Abca4-/- pigmented and albino mice were imaged to probe the fluorescence origin of NIR-AOFLIO. Results: The RPE mosaic was resolved at all locations in five of seven younger subjects (<35 years old). The mean lifetime across near-peripheral regions (8° and 12°) was longer compared to near-foveal regions (0° and 2°). Repeatability across two visits showed moderate to excellent correlation (intraclass correlation: 0.88 [τm], 0.75 [τ1], 0.65 [τ2], 0.98 [a1]). The mean lifetime across drusen-containing eyes was longer than in age-matched healthy eyes. Fluorescence was observed in only the extracts from pigmented Abca4-/- mouse. Conclusions: NIR-AOFLIO was repeatable and allowed visualization of the RPE cellular mosaic. An observed signal in only the pigmented mouse extract infers the fluorescence signal originates predominantly from melanin. Variations observed across the retina with intermediate age-related macular degeneration suggest NIR-AOFLIO may act as a functional measure of a biomarker for in vivo monitoring of early alterations in retinal health.
Databáze:	MEDLINE
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