Urinary Proteomic Biomarkers of Trabecular Bone Volume Change during Army Basic Combat Training.

Autor:	Flanagan SD, Hougland JR, Zeng X; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA., Cantrell PS; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA., Sun M; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA., Jones-Laughner J; Biomedical Mass Spectrometry Center, University of Pittsburgh, Pittsburgh, PA., Canino MC; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA., Hughes JM; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Foulis SA; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Taylor KM; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Walker LA; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Guerriere KI; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Sterczala AJ; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA., Connaboy C, Beckner ME; Military Performance Division, United States Army Research Institute of Environmental Medicine, Natick, MA., Matheny RW; Military Operational Medicine Research Program, Fort Detrick, MD., Nindl BC; Department of Sports Medicine and Nutrition, School of Health and Rehabilitation Sciences, University of Pittsburgh, Pittsburgh, PA.
Jazyk:	angličtina
Zdroj:	Medicine and science in sports and exercise [Med Sci Sports Exerc] 2024 Sep 01; Vol. 56 (9), pp. 1644-1654. Date of Electronic Publication: 2024 May 15.
DOI:	10.1249/MSS.0000000000003464
Abstrakt:	Purpose: The purpose of this study is to optimize a dMS-based urinary proteomic technique and evaluate the relationship between urinary proteome content and adaptive changes in bone microarchitecture during BCT. Methods: Urinary proteomes were analyzed with an optimized dMS technique in two groups of 13 recruits ( N = 26) at the beginning (Pre) and end (Post) of BCT. Matched by age (21 ± 4 yr), sex (16 W), and baseline tibial trabecular bone volume fractions (Tb.BV/TV), these groups were distinguished by the most substantial (High) and minimal (Low) improvements in Tb.BV/TV. Differential protein expression was analyzed with mixed permutation ANOVA and false discovery proportion-based adjustment for multiple comparisons. Results: Tibial Tb.BV/TV increased from pre- to post-BCT in High (3.30 ± 1.64%, P < 0.0001) but not Low (-0.35 ± 1.25%, P = 0.4707). The optimized dMS technique identified 10,431 peptides from 1368 protein groups that represented 165 integrative biological processes. Seventy-four urinary proteins changed from pre- to post-BCT ( P = 0.0019), and neutrophil-mediated immunity was the most prominent ontology. Two proteins (immunoglobulin heavy constant gamma 4 and C-type lectin domain family 4 member G) differed from pre- to post-BCT in High and Low ( P = 0.0006). Conclusions: The dMS technique can identify more than 1000 urinary proteins. At least 74 proteins are responsive to BCT, and other principally immune system-related proteins show differential expression patterns that coincide with adaptive bone formation. (Copyright © 2024 by the American College of Sports Medicine.)
Databáze:	MEDLINE
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