Terbium Phenanthroline Complex as a Luminescent Probe for the Detection of Anthrax Biomarker: Dipicolinic acid.

Autor: Abraham MK; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Chinnu SS; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Madanan AS; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Varghese S; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Shkhair AI; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Indongo G; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Rajeevan G; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., Arathy BK; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India., George S; Department of Chemistry, School of Physical and Mathematical Sciences, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala, 695581, India. emailtosony@gmail.com.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2024 May 17. Date of Electronic Publication: 2024 May 17.
DOI: 10.1007/s10895-024-03747-0
Abstrakt: Dipicolinic acid (DPA) is a prominent biomarker for Anthrax disease. Bacillus anthracis bacterial endospores is composed of DPA as the significant component, which on over inhalation can cause severe health issues. Such contagious and life-threatening pathogens can be employed as bioweapons or biothreat agents for spreading bioterrorism which is a major risk for national security and public health concerns. Hence, effective detection or a surveillance system is essential for preventing the growth of bioterrorism events. Herein, we have developed a Terbium - 1,10 Phenanthroline (Tb-Phen) based lanthanide luminescence complex with bright green fluorescence. On addition of DPA, the green fluorescence is turn-off at a linear range from 0.6 to 4.762 mM. In this effect, 5 D 4 - 7 F 5 transition caused by 1,10-phenanthroline to Tb 3+ at 544 nm is restricted due to energy transfer quenching and Inner Filter Effect (IFE). The developed probe shows good sensitivity towards the detection of DPA with other coexisting biomolecules and ions with a low Limit of Detection (LOD) of 5.029 µM. The practical feasibility was evaluated in paper strip assay and extended in real samples such as human serum and tap water with satisfactory recovery percentage. Thereby, probe finds promising application in sensing of anthrax spore biomarker (DPA) and biothreat agents.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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