Diffusion-driven fed-batch fermentation in perforated ring flasks.

Autor: Lüchtrath C; AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany., Lamping F; AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany., Hansen S; Evonik Operations GmbH, Paul-Baumann-Straße 1, 45772, Marl, Germany., Finger M; AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany., Magnus J; AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany., Büchs J; AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany. jochen.buechs@avt.rwth-aachen.de.
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2024 Aug; Vol. 46 (4), pp. 571-582. Date of Electronic Publication: 2024 May 17.
DOI: 10.1007/s10529-024-03493-0
Abstrakt: Purpose: Simultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring flask, which consists of a cylindrical glass flask with an additional perforated inner glass ring, for rapid bioprocess development.
Methods: A 3D-printed adapter was constructed to enable monitoring of the oxygen transfer rate in the perforated ring flasks. Escherichia coli experiments in batch were performed to validate the adapter. Fed-batch experiments with different diffusion rates and feed solutions were performed.
Results: The adapter and the performed experiments allowed a direct comparison of the perforated ring flasks with Erlenmeyer flasks. In batch cultivations, maximum oxygen transfer capacities of 80 mmol L -1  h -1 were reached with perforated ring flasks, corresponding to a 3.5 times higher capacity than in Erlenmeyer flasks. Fed-batch experiments with a feed reservoir concentration of 500 g glucose L -1 were successfully conducted. Based on the oxygen transfer rate, an ammonium limitation could be observed. By adding 40 g ammonium sulfate L -1 to the feed reservoir, the limitation could be prevented.
Conclusion: The membrane-based feeding, an online monitoring technique, and the perforated ring flask were successfully combined and offer a new and promising tool for screening and process development in biotechnology.
(© 2024. The Author(s).)
Databáze: MEDLINE
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