De Novo Genome Assembly for the Coppery Titi Monkey (Plecturocebus cupreus): An Emerging Nonhuman Primate Model for Behavioral Research.

Autor:	Pfeifer SP; School of Life Sciences, Arizona State University, Tempe, AZ, USA.; Center for Evolution and Medicine, Arizona State University, Tempe, AZ, USA., Baxter A; Department of Psychology, University of California, Davis, CA, USA.; California National Primate Research Center, Neuroscience and Behavior Division, Davis, CA, USA., Savidge LE; Department of Psychology, University of California, Davis, CA, USA.; California National Primate Research Center, Neuroscience and Behavior Division, Davis, CA, USA., Sedlazeck FJ; Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA., Bales KL; Department of Psychology, University of California, Davis, CA, USA.; California National Primate Research Center, Neuroscience and Behavior Division, Davis, CA, USA.; Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA, USA.
Jazyk:	angličtina
Zdroj:	Genome biology and evolution [Genome Biol Evol] 2024 May 02; Vol. 16 (5).
DOI:	10.1093/gbe/evae108
Abstrakt:	The coppery titi monkey (Plecturocebus cupreus) is an emerging nonhuman primate model system for behavioral and neurobiological research. At the same time, the almost entire absence of genomic resources for the species has hampered insights into the genetic underpinnings of the phenotypic traits of interest. To facilitate future genotype-to-phenotype studies, we here present a high-quality, fully annotated de novo genome assembly for the species with chromosome-length scaffolds spanning the autosomes and chromosome X (scaffold N50 = 130.8 Mb), constructed using data obtained from several orthologous short- and long-read sequencing and scaffolding techniques. With a base-level accuracy of ∼99.99% in chromosome-length scaffolds as well as benchmarking universal single-copy ortholog and k-mer completeness scores of >99.0% and 95.1% at the genome level, this assembly represents one of the most complete Pitheciidae genomes to date, making it an invaluable resource for comparative evolutionary genomics research to improve our understanding of lineage-specific changes underlying adaptive traits as well as deleterious mutations associated with disease. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.)
Databáze:	MEDLINE
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