Variation of terpene alkaloids in Daphniphyllum macropodum across plants and tissues.

Autor: Eljounaidi K; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK., Radzikowska BA; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK.; Department of Chemistry, University of York, York, YO10 5DD, UK., Whitehead CB; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK., Taylor DJ; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK., Conde S; Department of Biology, University of York, York, YO10 5DD, UK., Davis W; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK., Dowle AA; Biosciences Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., Langer S; Biosciences Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., James S; Biosciences Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., Unsworth WP; Department of Chemistry, University of York, York, YO10 5DD, UK., Ezer D; Department of Biology, University of York, York, YO10 5DD, UK., Larson TR; Biosciences Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., Lichman BR; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Jul; Vol. 243 (1), pp. 299-313. Date of Electronic Publication: 2024 May 17.
DOI: 10.1111/nph.19814
Abstrakt: Daphniphyllum macropodum produces alkaloids that are structurally complex with polycyclic, stereochemically rich carbon skeletons. Understanding how these compounds are formed by the plant may enable exploration of their biological function and bioactivities. We employed multiple metabolomics techniques, including a workflow to annotate compounds in the absence of standards, to compare alkaloid content across plants and tissues. Different alkaloid structural types were found to have distinct distributions between genotypes, between tissues and within tissues. Alkaloid structural types also showed different isotope labelling enrichments that matched their biosynthetic relationships. The work suggests that mevalonate derived 30-carbon alkaloids are formed in the phloem region before their conversion to 22-carbon alkaloids which accumulate in the epidermis. This sets the stage for further investigation into the biosynthetic pathway.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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