Periodontal pathogen Aggregatibacter actinomycetemcomitans JP2 correlates with colonic leukocytes decrease and gut microbiome imbalance in mice.

Autor: da Costa ALA; Oral Microbiology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.; Cellular Immunology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Soares MA; Department of Pharmaceutical Biotechnology, Laboratory of Studies in Experimental Pharmacology, Faculty of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Lourenço TGB; Oral Microbiology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Guimarães-Pinto K; Cellular Immunology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Filardy AD; Cellular Immunology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira AM; Oral Microbiology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Luca BG; Graduate Program of Pathology, Fluminense Federal University, Rio de Janeiro, Brazil., Magliano AC; Graduate Program of Pathology, Fluminense Federal University, Rio de Janeiro, Brazil., Araujo OMO; Laboratory of Nuclear Instrumentation, Nuclear Engineering Program, Institute Alberto Luiz de Coimbra of Graduate and Research in Engineering, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Moura L; Laboratory of Nuclear Instrumentation, Nuclear Engineering Program, Institute Alberto Luiz de Coimbra of Graduate and Research in Engineering, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Lopes RT; Laboratory of Nuclear Instrumentation, Nuclear Engineering Program, Institute Alberto Luiz de Coimbra of Graduate and Research in Engineering, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Palhares de Miranda AL; Cellular Immunology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Tributino JLM; Molecular Pharmacology Laboratory, Institute of Biomedical Sciences, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Vieira Colombo AP; Oral Microbiology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Journal of periodontal research [J Periodontal Res] 2024 Oct; Vol. 59 (5), pp. 961-973. Date of Electronic Publication: 2024 May 16.
DOI: 10.1111/jre.13288
Abstrakt: Aim: Evidence suggests that translocation of oral pathogens through the oral-gut axis may induce intestinal dysbiosis. This study aimed to evaluate the impact of a highly leukotoxic Aggregatibacter actinomycetemcomitans (Aa) strain on the gut microbiota, intestinal mucosal integrity and immune system in healthy mice.
Methods: Eight-week-old male C57BL6 mice were divided into control (n = 16) and JP2 groups (n = 19), which received intragastric gavage with PBS and with a suspension of Aa JP2 (HK921), respectively, twice a week for 4 weeks. Colonic lamina propria, fecal material, serum, gingival tissues, and mandibles were obtained for analyses of leukocyte populations, inflammatory mediators, mucosal integrity, alveolar bone loss, and gut microbiota. Differences between groups for these parameters were examined by non-parametric tests.
Results: The gut microbial richness and the number of colonic macrophages, neutrophils, and monocytes were significantly lower in Aa JP2-infected mice than in controls (p < .05). In contrast, infected animals showed higher abundance of Clostridiaceae, Lactobacillus taiwanensis, Helicobacter rodentium, higher levels of IL-6 expression in colonic tissues, and higher splenic MPO activity than controls (p < .05). No differences in tight junction expression, serum endotoxin levels, and colonic inflammatory cytokines were observed between groups. Infected animals presented also slightly more alveolar bone loss and gingival IL-6 levels than controls (p < .05).
Conclusion: Based on this model, intragastric administration of Aa JP2 is associated with changes in the gut ecosystem of healthy hosts, characterized by less live/recruited myeloid cells, enrichment of the gut microbiota with pathobionts and decrease in commensals. Negligible levels of colonic pro-inflammatory cytokines, and no signs of mucosal barrier disruption were related to these changes.
(© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: