Humane Endpoint: Example from a Murine Model of Disseminated Sporotrichosis.

Autor: Poester VR; Programa de Pós-Graduação Em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal Do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil. vanicerp@gmail.com.; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal Do Rio Grande, Campus Saúde. Visconde de Paranaguá 102, Centro, Rio Grande, RS, 96201-900, Brasil. vanicerp@gmail.com., Munhoz LS; Programa de Pós-Graduação Em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal Do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil.; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal Do Rio Grande, Campus Saúde. Visconde de Paranaguá 102, Centro, Rio Grande, RS, 96201-900, Brasil., Trápaga MR; Programa de Pós-Graduação Em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal Do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil.; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal Do Rio Grande, Campus Saúde. Visconde de Paranaguá 102, Centro, Rio Grande, RS, 96201-900, Brasil., Esperon BM; Programa de Pós-Graduação Em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal Do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil.; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal Do Rio Grande, Campus Saúde. Visconde de Paranaguá 102, Centro, Rio Grande, RS, 96201-900, Brasil., Stevens DA; California Institute for Medical Research, San Jose, USA. stevens@stanford.edu.; Division of Infectious Diseases and Geographic Medicine, Stanford University Medical School, Stanford, USA. stevens@stanford.edu.; Foundation for Research in Infectious Diseases, P.O. Box 2734, Saratoga, 95070, USA. stevens@stanford.edu., Xavier MO; Programa de Pós-Graduação Em Ciências da Saúde, Faculdade de Medicina (FAMED), Universidade Federal Do Rio Grande (FURG), Rio Grande, Rio Grande do Sul (RS), Brazil. melissaxavierfurg@gmail.com.; Laboratório de Micologia, Faculdade de Medicina, Universidade Federal Do Rio Grande, Campus Saúde. Visconde de Paranaguá 102, Centro, Rio Grande, RS, 96201-900, Brasil. melissaxavierfurg@gmail.com.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 May 16; Vol. 81 (7), pp. 175. Date of Electronic Publication: 2024 May 16.
DOI: 10.1007/s00284-024-03692-y
Abstrakt: Vertebrate animal models are essential in research; however, efforts need to be made to decrease animal suffering as much as possible. It could be useful to determine humane endpoints that could serve as surrogates for a fatal outcome. We address this issue with respect to infectious diseases. We propose a humane endpoint for studies of Sporothrix brasiliensis infection. BALB/c mice were inoculated subcutaneously in the footpad. To define a humane endpoint, we considered two groups: animals who died during the experiment, and those euthanized at the end of the experiment. The groups were compared for colony-forming units (CFU) in internal organs, clinical condition, and body weight. Thirteen (22%) animals died before the end of the experiment due to the progression of local infection to severe and disseminated sporotrichosis. Analyzing data of the groups, we propose the following future criteria for euthanasia as a humane endpoint: neurological impairment OR necrosis of the footpad OR loss of ≥ 20% body weight AND moderate to severe dehydration. In view of the current global epidemiological impact of zoonotic sporotrichosis caused by S. brasiliensis, our data could facilitate the utility of models used to study the disease, particularly therapy.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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