Homocysteine thiolactone and other sulfur-containing amino acid metabolites are associated with fibrin clot properties and the risk of ischemic stroke.

Autor: Sikora M; European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, 61-704, Poznań, Poland., Bretes E; Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632, Poznań, Poland., Perła-Kaján J; Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632, Poznań, Poland., Utyro O; Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632, Poznań, Poland., Borowczyk K; Faculty of Chemistry, Department of Environmental Chemistry, University of Łódź, 90-236, Łódź, Poland., Piechocka J; Faculty of Chemistry, Department of Environmental Chemistry, University of Łódź, 90-236, Łódź, Poland., Głowacki R; Faculty of Chemistry, Department of Environmental Chemistry, University of Łódź, 90-236, Łódź, Poland., Wojtasz I; Medicover, 61-131, Poznań, Poland., Kaźmierski R; Department of Neurology, Collegium Medicum, University of Zielona Góra, 65-046, Zielona Góra, Poland.; Department of Neurology, Poznań University of Medical Sciences, 60-355, Poznań, Poland., Jakubowski H; Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, 60-632, Poznań, Poland. jakubows@rutgers.edu.; Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, International Center for Public Health, 225 Warren Street, Newark, NJ, 07103, USA. jakubows@rutgers.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 16; Vol. 14 (1), pp. 11222. Date of Electronic Publication: 2024 May 16.
DOI: 10.1038/s41598-024-60706-2
Abstrakt: Homocysteine (Hcy) and Hcy-thiolactone (HTL) affect fibrin clot properties and are linked to cardiovascular disease. Factors that influence fibrin clot properties and stroke are not fully understood. To study sulfur-containing amino acid metabolites, fibrin clot lysis time (CLT) and maximum absorbance (Abs max ) in relation to stroke, we analyzed plasma and urine from 191 stroke patients (45.0% women, age 68 ± 12 years) and 291 healthy individuals (59.7% women, age 50 ± 17 years). Plasma and urinary levels of sulfur-containing amino acid metabolites and fibrin clot properties were significantly different in stroke patients compared to healthy individuals. Fibrin CLT correlated with fibrin Abs max in healthy males (R 2  = 0.439, P = 0.000), females (R 2  = 0.245, P = 0.000), female stroke patients (R 2  = 0.187, P = 0.000), but not in male stroke patients (R 2  = 0.008, P = ns). Fibrin CLT correlated with age in healthy females but not males while fibrin Abs max correlated with age in both sexes; these correlations were absent in stroke patients. In multiple regression analysis in stroke patients, plasma (p)CysGly, pMet, and MTHFR A1298C polymorphism were associated with fibrin Abs max , while urinary (u)HTL, uCysGly, and pCysGly were significantly associated with fibrin CLT. In healthy individuals, uHTL and uGSH were significantly associated with fibrin Abs max , while pGSH, and CBS T833C 844ins68 polymorphism were associated with fibrin CLT. In logistic regression, uHTL, uHcy, pCysGly, pGSH, MTHFR C677T polymorphism, and Abs max were independently associated with stroke. Our findings suggest that HTL and other sulfur-containing amino acid metabolites influence fibrin clot properties and the risk of stroke.
(© 2024. The Author(s).)
Databáze: MEDLINE
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