Inactivation mechanism of phenyllactic acid against Bacillus cereus spores and its application in milk beverage.

Autor: Zhang D; College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Tong D; College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Wang Z; College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Wang S; College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Jia Y; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China., Ning Y; College of Food and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China. Electronic address: ningyw@hebust.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Sep 30; Vol. 453, pp. 139601. Date of Electronic Publication: 2024 May 11.
DOI: 10.1016/j.foodchem.2024.139601
Abstrakt: Phenyllactic acid (PLA) as a natural phenolic acid exhibits antibacterial activity against non-spore-forming bacteria, while the inhibitory effect against bacterial spore remained unknown. Herein, this study investigated the inactivation effect of PLA against Bacillus cereus spores. The results revealed that the minimum inhibitory concentration of PLA was 1.25 mg/mL. PLA inhibited the outgrowth of germinated spores into vegetative cells rather than germination of spores. PLA disrupted the spore coat, and damaged the permeability and integrity of inner membrane. Moreover, PLA disturbed the establishment of membrane potential due to the inhibition of oxidative metabolism. SEM observations further visualized the morphological changes and structural disruption caused by PLA. Besides, PLA caused the degradation of DNA of germinated spores. Finally, PLA was applied in milk beverage, and showed promising inhibitory effect against B. cereus spores. This finding could provide scientific basis for the application of PLA against spore-forming bacteria in food industry.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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