Chromosome analysis of 303 pregnancy losses in Mexico.

Autor:	Garduño-Zarazúa LM; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico.; Human Genetics Medical Research Unit, High Specialty Medical Unit, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City.; Biomedical Sciences Doctorate Program, Universidad Nacional Autónoma de México, Mexico City., Mayén DG; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico., Meléndez-Hernández R; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico., Paz-Martínez AJ; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico., Ramírez-Arroyo E; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico., Sosa D; Applied Genetics Unit, Hospital Ángeles Lomas, Huixquilucan, State of Mexico., Aguinaga M; Department of Genetics, Instituto Nacional de Perinatología, Mexico City., Pérez-Durán J; Department of Reproductive and Perinatal Health Research, Instituto Nacional de Perinatología, Mexico City., Ríos O; Citogenetics Laboratory, Instituto Nacional de Pediatría, Mexico City., López-Valdez J; Department of Genetics, Hospital Centenario Miguel Hidalgo, Aguascalientes, Aguascalientes.; Woman's Hospital, Instituto de Salud del Estado de Aguascalientes, Aguascalientes., Torres L; Citogenetics Laboratory, Instituto Nacional de Pediatría, Mexico City., Frias S; Citogenetics Laboratory, Instituto Nacional de Pediatría, Mexico City.; Department of Genomic Medicine and Environmental Toxicology, Institute of Biomedical Research, Universidad Nacional Autónoma de México, Mexico City. Mexico.
Jazyk:	angličtina
Zdroj:	Gaceta medica de Mexico [Gac Med Mex] 2024; Vol. 160 (1), pp. 76-85.
DOI:	10.24875/GMM.M24000859
Abstrakt:	Background: Chromosomal abnormalities are present in 50 to 60% of miscarriages and in 6 to 19% of stillbirths. Although microarrays are preferred for studying chromosomal abnormalities, many hospitals cannot offer this methodology. Objective: To present the results of the cytogenetic analysis of 303 products of conception (POC), which included 184 miscarriages, 49 stillbirths and 17 cases of undefined age. Material and Methods: Karyotyping, fluorescence in situ hybridization, short tandem repeats and microarrays were used, depending on the type of loss and available sample. Results: In 29 POCs we found maternal tissue and were eliminated from the analyses. Informative results were obtained in 250 (91.2 %)/274 cases; the karyotyping success rate was 80.7%; that of single nucleotide polymorphism microarrays, 94.5%; and that of fluorescence in situ hybridization and short tandem repeat, 100%. Cytogenetic abnormalities were observed in 57.6% of miscarriages and in 24.5% of stillbirths; 94% of total anomalies were numerical and 6% were submicroscopic. Conclusions: Karyotyping with simultaneous short tandem repeat study to rule out contamination of maternal cells is effective for studying miscarriages; in stillbirths, microarrays are recommended. (Copyright: © 2024 Permanyer.)
Databáze:	MEDLINE
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