Detection and genetic analysis of bovine rhinitis B virus in Japan.

Autor: Ishida H; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan. ishida@azabu-u.ac.jp., Nakamura M; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan., Murakami H; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan., Kazama K; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan., Oba M; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.; Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Takemae H; Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Mizutani T; Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Ouchi Y; Beef Cattle Institute, Ibaraki Prefecture of Livestock Research Center, Hitachi-Omiya, Ibaraki, Japan., Kawakami J; Ibaraki Prefecture Kennan Livestock Hygiene Service Center, Tsuchiura, Ibaraki, Japan., Tsuzuku S; Ibaraki Prefecture Kennan Livestock Hygiene Service Center, Tsuchiura, Ibaraki, Japan., Nagai M; School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 May 16; Vol. 169 (6), pp. 125. Date of Electronic Publication: 2024 May 16.
DOI: 10.1007/s00705-024-06046-y
Abstrakt: Bovine rhinitis B virus (BRBV) (genus Aphthovirus, family Picornaviridae) is a significant etiological agent of the bovine respiratory disease complex. Despite global reports on BRBV, genomic data for Japanese strains are not available. In this study, we aimed to obtain genomic information on BRBV in Japan and analyze its genetic characteristics. In nasal swabs from 66 cattle, BRBV was detected in 6 out of 10 symptomatic and 4 out of 56 asymptomatic cattle. Using metagenomic sequencing and Sanger sequencing, the nearly complete genome sequences of two Japanese BRBV strains, IBA/2211/2 and LAV/238002, from symptomatic and asymptomatic cattle, respectively, were determined. These viruses shared significant genetic similarity with known BRBV strains and exhibited unique mutations and recombination events, indicating dynamic evolution, influenced by regional environmental and biological factors. Notably, the leader gene was only approximately 80% and 90% identical in its nucleotide and amino acid sequence, respectively, to all of the BRBV strains with sequences in the GenBank database, indicating significant genetic divergence in the Japanese BRBV leader gene. These findings provide insights into the genetic makeup of Japanese BRBV strains, enriching our understanding of their genetic diversity and evolutionary mechanisms.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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