Development of a Gaussia luciferase immunoprecipitation assay for detecting Schistosoma japonicum infection.

Autor: Wang X; Shanghai Tenth People's Hospital, Institute for Infectious Diseases and Vaccine Development, Tongji University School of Medicine, Shanghai, 200331, China; School of Biotechnology Jiangsu University of Science and Technology, Zhen Jiang, 212100, China., Giri BR; Department of Zoology, Utkal University, Bhubaneswar, Odisha, 751004, India., Cui Z; Pingquan Hospital, Hebei Province, Chengde, 067500, China., Munkhjargal T; Institute of Veterinary Medicine, Mongolian University of Life Science, Zaisan, 17029, Ulaanbaatar, Mongolia., Wang C; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang Province, Daqing, 163316, China., Fontanilla IKC; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines., Cheng G; Shanghai Tenth People's Hospital, Institute for Infectious Diseases and Vaccine Development, Tongji University School of Medicine, Shanghai, 200331, China. Electronic address: cheng_guofeng@foxmail.com.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2024 Jul; Vol. 262, pp. 108776. Date of Electronic Publication: 2024 May 14.
DOI: 10.1016/j.exppara.2024.108776
Abstrakt: Timely and accurate diagnosis of Schistosoma infection is important to adopt effective strategies for schistosomiasis control. Previously, we demonstrated that Schistosoma japonicum can secret extracellular vesicles and their cargos may serve as a novel type of biomarkers for diagnosing schistosomiasis. Here, we developed a Gaussia luciferase immunoprecipitation assay combined with S. japonicum extracellular vesicle (SjEV) protein to evaluate its potential for diagnosing schistosomiasis. A saposin-like protein (SjSLP) identified from SjEVs was fused to the Gaussia luciferase as the diagnostic antigen. The developed method showed good capability for detecting S. japonicum infection in mice and human patients. We also observed that the method could detect Schistosoma infection in mice as early as 7 days of post-infection, which showed better sensitivity than that of indirect ELISA method. Overall, the developed method showed a good potential for detecting Schistosoma infection particularly for early stage, which may provide an alternative strategy for identify Schistosoma infection for disease control.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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