Development of a novel laboratory photodynamic therapy device: automated multi-mode LED system for optimum well-plate irradiation.

Autor: Yildiz MZ; Faculty of Technology, Electrical-Electronics Engineering, Sakarya University of Applied Sciences, Serdivan, Turkey., Kamanli AF; Biomedical Technologies Application and Research Center (Biyotam), Sakarya University of Applied Sciences, Serdivan, Turkey. fkamanli@sakarya.edu.tr., Eskiler GG; Faculty of Medicine, Sakarya University, Serdivan, Turkey., Tabakoğlu HÖ; Biomedical Engineering, Bakircay University, Izmir, Turkey., Pala MA; Health Services Vocational School, Sakarya University, Serdivan, Sakarya, Turkey., Özdemir AE; Health Services Vocational School, Sakarya University, Serdivan, Sakarya, Turkey.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2024 May 16; Vol. 39 (1), pp. 131. Date of Electronic Publication: 2024 May 16.
DOI: 10.1007/s10103-024-04083-2
Abstrakt: Photodynamic therapy (PDT) is a targeted treatment method that utilizes a photosensitizer (PS) to induce cytotoxicity in malignant and non-malignant tumors. Optimization of PDT requires investigation of the selectivity of PS for the target tissues, irradiating light source, irradiation wavelengths, fluence rate, fluence, illumination mode, and overall treatment plan. In this study, we developed the Multi-mode Automatized Well-plate PDT LED Laboratory Irradiation System (MAWPLIS), an innovative device that automates time-consuming well plate light dosage/PS dose measurement experiment. The careful control of LED current and temperature stabilization in the LED module allowed the system to achieve high optical output stability. The MAWPLIS was designed by integrating a 3-axis moving system and motion controller, a quick-switching LED controller unit equipped with interchangeable LED modules capable of employing multiple wavelengths, and a TEC system. The proposed system achieved high optical output stability (1 mW) within the range of 0-500 mW, high wavelength stability (5 nm) at 635 nm, and high temperature stability (0.2 °C) across all radiation modes. The system's validation involved in vitro analysis using 5-ALA across varying concentrations, incubation periods, light exposures, and wavelengths in HT-29 colon cancer and WI-38 human lung fibroblast cell lines. Specifically, a combination of 405 nm and 635 nm wavelengths was selected to demonstrate enhanced strategies for colon cancer cell eradication and system validation. The MAWPLIS system represents a significant advancement in photodynamic therapy (PDT) research, offering automation and standardization of time-intensive experiments, high stability and precision, and improved PDT efficacy through dual-wavelength integration.
(© 2024. The Author(s).)
Databáze: MEDLINE
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