An immunohistochemical atlas of necroptotic pathway expression.

Autor:	Chiou S; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Al-Ani AH; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia.; Royal Melbourne Hospital, Parkville, Australia., Pan Y; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Patel KM; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Kong IY; Pediatric Hematology/Oncology, Weill Cornell Medical College, New York, USA., Whitehead LW; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Light A; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Young SN; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Barrios M; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Sargeant C; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Rajasekhar P; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Zhu L; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Hempel A; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Lin A; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Rickard JA; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; Austin Hospital, Heidelberg, Australia., Hall C; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Gangatirkar P; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Yip RK; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Cawthorne W; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Jacobsen AV; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Horne CR; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Martin KR; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Ioannidis LJ; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Hansen DS; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia.; Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Clayton, Australia., Day J; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia.; Royal Melbourne Hospital, Parkville, Australia., Wicks IP; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Law C; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Ritchie ME; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Bowden R; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Hildebrand JM; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., O'Reilly LA; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Silke J; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Giulino-Roth L; Pediatric Hematology/Oncology, Weill Cornell Medical College, New York, USA., Tsui E; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Rogers KL; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Hawkins ED; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia., Christensen B; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.; University of Melbourne, Parkville, Australia.; Royal Melbourne Hospital, Parkville, Australia., Murphy JM; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia. jamesm@wehi.edu.au.; University of Melbourne, Parkville, Australia. jamesm@wehi.edu.au.; Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia. jamesm@wehi.edu.au., Samson AL; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia. samson.a@wehi.edu.au.; University of Melbourne, Parkville, Australia. samson.a@wehi.edu.au.
Jazyk:	angličtina
Zdroj:	EMBO molecular medicine [EMBO Mol Med] 2024 Jul; Vol. 16 (7), pp. 1717-1749. Date of Electronic Publication: 2024 May 15.
DOI:	10.1038/s44321-024-00074-6
Abstrakt:	Necroptosis is a lytic form of regulated cell death reported to contribute to inflammatory diseases of the gut, skin and lung, as well as ischemic-reperfusion injuries of the kidney, heart and brain. However, precise identification of the cells and tissues that undergo necroptotic cell death in vivo has proven challenging in the absence of robust protocols for immunohistochemical detection. Here, we provide automated immunohistochemistry protocols to detect core necroptosis regulators - Caspase-8, RIPK1, RIPK3 and MLKL - in formalin-fixed mouse and human tissues. We observed surprising heterogeneity in protein expression within tissues, whereby short-lived immune barrier cells were replete with necroptotic effectors, whereas long-lived cells lacked RIPK3 or MLKL expression. Local changes in the expression of necroptotic effectors occurred in response to insults such as inflammation, dysbiosis or immune challenge, consistent with necroptosis being dysregulated in disease contexts. These methods will facilitate the precise localisation and evaluation of necroptotic signaling in vivo. (© 2024. The Author(s).)
Databáze:	MEDLINE
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