The role of glial and neuronal Eph/ephrin signaling in Drosophila mushroom body development and sleep and circadian behavior.

Autor: Lee JE; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea., Lee H; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea., Baek E; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea., Choi B; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea., Yun HS; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea., Yoo YK; Department of Electronic Engineering, Catholic Kwandong University, Gangneung, Gangwon-do, 25601, Republic of Korea., Lee YS; Department of Medical Science, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do, 25601, Republic of Korea; Translational Brain Research Center, International St. Mary's Hospital, Catholic Kwandong University, Incheon, 22711, Republic of Korea., Song GJ; Department of Medical Science, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do, 25601, Republic of Korea; Translational Brain Research Center, International St. Mary's Hospital, Catholic Kwandong University, Incheon, 22711, Republic of Korea., Cho KS; Department of Biological Sciences, Konkuk University, Seoul, 05029, Republic of Korea; Korea Hemp Institute, Konkuk University, Seoul, 05029, Republic of Korea. Electronic address: kscho@konkuk.ac.kr.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Aug 06; Vol. 720, pp. 150072. Date of Electronic Publication: 2024 May 10.
DOI: 10.1016/j.bbrc.2024.150072
Abstrakt: The Eph receptor, a prototypically large receptor protein tyrosine kinase, interacts with ephrin ligands, forming a bidirectional signaling system that impacts diverse brain functions. Eph receptors and ephrins mediate forward and reverse signaling, affecting neurogenesis, axon guidance, and synaptic signaling. While mammalian studies have emphasized their roles in neurogenesis and synaptic plasticity, the Drosophila counterparts are less studied, especially in glial cells, despite structural similarities. Using RNAi to modulate Eph/ephrin expression in Drosophila neurons and glia, we studied their roles in brain development and sleep and circadian behavior. Knockdown of neuronal ephrin disrupted mushroom body development, while glial knockdown had minimal impact. Surprisingly, disrupting ephrin in neurons or glial cells altered sleep and circadian rhythms, indicating a direct involvement in these behaviors independent from developmental effects. Further analysis revealed distinct sleep phenotypes between neuronal and glial knockdowns, underscoring the intricate interplay within the neural circuits that govern behavior. Glia-specific knockdowns showed altered sleep patterns and reduced circadian rhythmicity, suggesting an intricate role of glia in sleep regulation. Our findings challenge simplistic models of Eph/ephrin signaling limited to neuron-glia communication and emphasize the complexity of the regulatory networks modulating behavior. Future investigations targeting specific glial subtypes will enhance our understanding of Eph/ephrin signaling's role in sleep regulation across species.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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