Synergistic induction of blood-brain barrier properties.

Autor:	Porkoláb G; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary.; Doctoral School of Biology, University of Szeged, Szeged H-6720, Hungary., Mészáros M; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Szecskó A; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary.; Doctoral School of Biology, University of Szeged, Szeged H-6720, Hungary., Vigh JP; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary.; Doctoral School of Biology, University of Szeged, Szeged H-6720, Hungary., Walter FR; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Figueiredo R; GenXPro GmbH, Frankfurt am Main 60438, Germany., Kálomista I; In Vitro Metabolism Laboratory, Gedeon Richter, Budapest H-1103, Hungary., Hoyk Z; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Vizsnyiczai G; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Gróf I; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Jan JS; Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan., Gosselet F; Laboratoire de la Barriére Hémato-Encéphalique, Université d'Artois, Lens 62307, France., Pirity MK; Institute of Genetics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Vastag M; In Vitro Metabolism Laboratory, Gedeon Richter, Budapest H-1103, Hungary., Hudson N; Smurfit Institute of Genetics, Trinity College Dublin, Dublin D02 VF25, Ireland., Campbell M; Smurfit Institute of Genetics, Trinity College Dublin, Dublin D02 VF25, Ireland., Veszelka S; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary., Deli MA; Institute of Biophysics, Biological Research Centre, Hungarian Research Network, Szeged H-6726, Hungary.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 May 21; Vol. 121 (21), pp. e2316006121. Date of Electronic Publication: 2024 May 15.
DOI:	10.1073/pnas.2316006121
Abstrakt:	Blood-brain barrier (BBB) models derived from human stem cells are powerful tools to improve our understanding of cerebrovascular diseases and to facilitate drug development for the human brain. Yet providing stem cell-derived endothelial cells with the right signaling cues to acquire BBB characteristics while also retaining their vascular identity remains challenging. Here, we show that the simultaneous activation of cyclic AMP and Wnt/β-catenin signaling and inhibition of the TGF-β pathway in endothelial cells robustly induce BBB properties in vitro. To target this interaction, we present a small-molecule cocktail named cARLA, which synergistically enhances barrier tightness in a range of BBB models across species. Mechanistically, we reveal that the three pathways converge on Wnt/β-catenin signaling to mediate the effect of cARLA via the tight junction protein claudin-5. We demonstrate that cARLA shifts the gene expressional profile of human stem cell-derived endothelial cells toward the in vivo brain endothelial signature, with a higher glycocalyx density and efflux pump activity, lower rates of endocytosis, and a characteristic endothelial response to proinflammatory cytokines. Finally, we illustrate how cARLA can improve the predictive value of human BBB models regarding the brain penetration of drugs and targeted nanoparticles. Due to its synergistic effect, high reproducibility, and ease of use, cARLA has the potential to advance drug development for the human brain by improving BBB models across laboratories. Competing Interests: Competing interests statement:I.K. and M.V. are employed by the company Gedeon Richter. HUN-REN BRC has filed a patent (PCT/HU2023/050070) related to this work; G.P., M.M., A.S., S.V., and M.A.D. are named inventors. Other authors declare no competing interest. Hungarian national patent P2300053 was filed on 9 February 2023 by HUN-REN Biological Research Centre, Szeged, Hungary. Inventors are authors G.P., M.A.D., S.V., M.M., and A.S.
Databáze:	MEDLINE
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