OMIP-103: A 35-marker imaging mass cytometry panel for the co-detection of HIV and immune cell populations in human formalin fixed paraffin embedded intestinal tissue.

Autor: Hu K; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia., O'Neil T; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia., Canete N; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia., Baharlou H; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia., Harman A; Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia.
Jazyk: angličtina
Zdroj: Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2024 Jul; Vol. 105 (7), pp. 488-492. Date of Electronic Publication: 2024 May 15.
DOI: 10.1002/cyto.a.24847
Abstrakt: We introduce a 35-marker imaging mass cytometry (IMC) panel for a detailed examination of immune cell populations and HIV RNA in formalin fixed paraffin embedded (FFPE) human intestinal tissue. The panel has broad cell type coverage and particularly excels in delineating subsets of mononuclear phagocytes and T cells. Markers for key tissue structures are included, enabling identification of epithelium, blood vessels, lymphatics, and musculature. The described method for HIV RNA detection can be generalized to other low abundance RNA targets, whether endogenous or pathogen derived. As such, the panel presented here is useful for high parameter spatial mapping of intestinal immune cells and their interactions with pathogens such as HIV.
(© 2024 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.)
Databáze: MEDLINE
Externí odkaz: