High percentage of midpiece defects in Brangus bull sperm with no reduction in sperm kinematics.

Autor: Maggi G; Grupo Fisiopatologia e Biotécnicas da Reprodução Animal (FiBRA-UFPel), Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Brasil., Silveira D; Progen Inseminação Artificial, Dom Pedrito, Brasil., Vieira MB; Progen Inseminação Artificial, Dom Pedrito, Brasil., da Silva IGM; Laboratório de Microscopia e Microanálise, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Brasil., Barros-Cordeiro KB; Laboratório de Microscopia e Microanálise, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Brasil., Severo NC; NCSevero Consultoria Técnica, Uberaba, Brasil., Báo SN; Laboratório de Microscopia e Microanálise, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Brasil., Mondadori RG; Instituto de Biologia, Grupo Fisiopatologia e Biotécnicas da Reprodução Animal (FiBRA-UFPel), Universidade Federal de Pelotas, RS, Brasil.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 May; Vol. 59 (5), pp. e14585.
DOI: 10.1111/rda.14585
Abstrakt: The study investigated midpiece defects in sperm from a 5-year-old Brangus bull with a high rate of semen batch rejection, due to morphologically abnormal sperm, with no reduction in sperm kinematics. A comprehensive evaluation was conducted over a 16-month period, involving 28 ejaculates. Notably, despite the high proportion of midpiece defects (average 37.73%, from 3% to 58%), the study revealed stable sperm production, with no discernible differences in the kinematic data before and after cryopreservation. Electron microscopy identified discontinuities in the mitochondrial sheath, characteristic of midpiece aplasia (MPA). The anomalies were attributed to be of genetic origin, as other predisposing factors were absent. Additionally, the electron microscopy unveiled plasma membrane defects, vacuoles and chromatin decondensation, consistent with previous findings linking acrosome abnormalities with midpiece defects. The findings underscored the necessity of conducting thorough laboratory evaluations before releasing cryopreserved semen for commercialization. Despite substantial morphological alterations, the initial semen evaluation data indicated acceptable levels of sperm kinematics, emphasizing the resilience of sperm production to severe morphological changes. This case report serves as a contribution to the understanding of midpiece defects in bull sperm, emphasizing the need for meticulous evaluation and quality control in semen processing and commercialization.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: