Cows with diverging haplotypes show differences in differential milk cell count, milk parameters and vaginal temperature after S. aureus challenge but not after E. coli challenge.

Autor: Müller-Langhans K; Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany., Oberberger L; Institute for Infectious Diseases and Zoonoses, Ludwig-Maximilians-University Munich, Sonnenstrasse 24, Oberschleissheim, 85764, Germany., Zablotski Y; Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany., Engelmann S; Technical University Braunschweig, Institute for Microbiology, Inhoffenstrasse 7, Brunswick, 38124, Germany.; Helmholtz Center for Infection Research, Microbial Proteomics, Inhoffenstrasse 7, Brunswick, 38124, Germany., Hoedemaker M; Clinic for Cattle, University of Veterinary Medicine Hanover Foundation, Bischofsholer Damm 15, Hanover, 30173, Germany., Kühn C; Research Institute for Farm Animal Biology, Genome Biology, Wilhelm-Stahl-Allee 2, Dummerstorf, 18196, Germany.; Agricultural and Environmental Faculty, University Rostock, Justus-Von-Liebig-Weg 6, Rostock, 18059, Germany.; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Greifswald-Insel Riems, 17493, Germany., Schuberth HJ; Institute for Immunology, University of Veterinary Medicine Hanover Foundation, Bünteweg 2, Hanover, 30559, Germany., Zerbe H; Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany., Petzl W; Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany., Meyerholz-Wohllebe MM; Clinic for Ruminants With Ambulatory Clinic and Herd Health Services, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, Sonnenstrasse 16, Oberschleissheim, 85764, Germany. marie.meyerholz@lmu.de.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 May 15; Vol. 20 (1), pp. 200. Date of Electronic Publication: 2024 May 15.
DOI: 10.1186/s12917-024-03996-y
Abstrakt: Background: In dairy cattle, mastitis causes high financial losses and impairs animal well-being. Genetic selection is used to breed cows with reduced mastitis susceptibility. Techniques such as milk cell flow cytometry may improve early mastitis diagnosis. In a highly standardized in vivo infection model, 36 half-sib cows were selected for divergent paternal Bos taurus chromosome 18 haplotypes (Q vs. q) and challenged with Escherichia coli for 24 h or Staphylococcus aureus for 96 h, after which the samples were analyzed at 12 h intervals. Vaginal temperature (VT) was recorded every three minutes. The objective of this study was to compare the differential milk cell count (DMCC), milk parameters (fat %, protein %, lactose %, pH) and VT between favorable (Q) and unfavorable (q) haplotype cows using Bayesian models to evaluate their potential as improved early indicators of differential susceptibility to mastitis.
Results: After S. aureus challenge, compared to the Q half-sibship cows, the milk of the q cows exhibited higher PMN levels according to the DMCC (24 h, p < 0.001), a higher SCC (24 h, p < 0.01 and 36 h, p < 0.05), large cells (24 h, p < 0.05) and more dead (36 h, p < 0.001) and live cells (24 h, p < 0.01). The protein % was greater in Q milk than in q milk at 0 h (p = 0.025). In the S. aureus group, Q cows had a greater protein % (60 h, p = 0.048) and fat % (84 h, p = 0.022) than q cows. Initially, the greater VT of S. aureus-challenged q cows (0 and 12-24 h, p < 0.05) reversed to a lower VT in q cows than in Q cows (48-60 h, p < 0.05). Additionally, the following findings emphasized the validity of the model: in the S. aureus group all DMCC subpopulations (24 h-96 h, p < 0.001) and in the E. coli group nearly all DMCC subpopulations (12 h-24 h, p < 0.001) were higher in challenged quarters than in unchallenged quarters. The lactose % was lower in the milk samples of E. coli-challenged quarters than in those of S. aureus-challenged quarters (24 h, p < 0.001). Between 12 and 18 h, the VT was greater in cows challenged with E. coli than in those challenged with S. aureus (3-h interval approach, p < 0.001).
Conclusion: This in vivo infection model confirmed specific differences between Q and q cows with respect to the DMCC, milk component analysis results and VT results after S. aureus inoculation but not after E. coli challenge. However, compared with conventional milk cell analysis monitoring, e.g., the global SCC, the DMCC analysis did not provide refined phenotyping of the pathogen response.
(© 2024. The Author(s).)
Databáze: MEDLINE
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