Alpha-glucans from bacterial necromass indicate an intra-population loop within the marine carbon cycle.

Autor: Beidler I; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany., Steinke N; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany.; University of Bremen, Center for Marine Environmental Sciences, MARUM, 28359, Bremen, Germany., Schulze T; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany., Sidhu C; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany., Bartosik D; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany.; Institute of Marine Biotechnology, 17489, Greifswald, Germany., Zühlke MK; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany., Martin LT; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany., Krull J; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany.; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany., Dutschei T; Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17489, Greifswald, Germany., Ferrero-Bordera B; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Rielicke J; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Kale V; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Sura T; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Trautwein-Schult A; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Kirstein IV; Alfred Wegener Institute for Polar and Marine Research, Biologische Anstalt Helgoland, 27483, Helgoland, Germany., Wiltshire KH; Alfred Wegener Institute for Polar and Marine Research, Biologische Anstalt Helgoland, 27483, Helgoland, Germany., Teeling H; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany., Becher D; Microbial Proteomics, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Bengtsson MM; Microbial Physiology and Molecular Biology, Institute of Microbiology, University of Greifswald, 17489, Greifswald, Germany., Hehemann JH; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany.; University of Bremen, Center for Marine Environmental Sciences, MARUM, 28359, Bremen, Germany., Bornscheuer UT; Biotechnology and Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17489, Greifswald, Germany., Amann RI; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany., Schweder T; Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, 17489, Greifswald, Germany. schweder@uni-greifswald.de.; Institute of Marine Biotechnology, 17489, Greifswald, Germany. schweder@uni-greifswald.de.; Alfred Wegener Institute for Polar and Marine Research, Biologische Anstalt Helgoland, 27483, Helgoland, Germany. schweder@uni-greifswald.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 May 14; Vol. 15 (1), pp. 4048. Date of Electronic Publication: 2024 May 14.
DOI: 10.1038/s41467-024-48301-5
Abstrakt: Phytoplankton blooms provoke bacterioplankton blooms, from which bacterial biomass (necromass) is released via increased zooplankton grazing and viral lysis. While bacterial consumption of algal biomass during blooms is well-studied, little is known about the concurrent recycling of these substantial amounts of bacterial necromass. We demonstrate that bacterial biomass, such as bacterial alpha-glucan storage polysaccharides, generated from the consumption of algal organic matter, is reused and thus itself a major bacterial carbon source in vitro and during a diatom-dominated bloom. We highlight conserved enzymes and binding proteins of dominant bloom-responder clades that are presumably involved in the recycling of bacterial alpha-glucan by members of the bacterial community. We furthermore demonstrate that the corresponding protein machineries can be specifically induced by extracted alpha-glucan-rich bacterial polysaccharide extracts. This recycling of bacterial necromass likely constitutes a large-scale intra-population energy conservation mechanism that keeps substantial amounts of carbon in a dedicated part of the microbial loop.
(© 2024. The Author(s).)
Databáze: MEDLINE
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