Rapid quantification models for assessing melamine adulteration in sport nutrition supplements via benchtop and portable NIRS instruments.

Autor: Shutevska K; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia. Electronic address: k.sutevska@ff.ukim.edu.mk., Bajatovska AM; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia., Anastasova L; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia., Zhivikj Z; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia., Zafirova Gjorgievska M; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia., Spasikj S; Center for Public Health - Kumanovo, 11 Oktomvri bb, 1300, Kumanovo, Republic of North Macedonia., Petreska Ivanovska T; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia., Makreski P; Ss Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, Arhimedova 5, 1000, Skopje, Republic of North Macedonia., Geskovski N; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Majka Tereza 47, 1000, Skopje, Republic of North Macedonia. Electronic address: ngeskovski@ff.ukim.edu.mk.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Sep 05; Vol. 317, pp. 124370. Date of Electronic Publication: 2024 May 01.
DOI: 10.1016/j.saa.2024.124370
Abstrakt: Sport nutrition supplements (SNS) are vulnerable to adulteration with melamine, artificially augmenting their protein content as determined by conventional assay methodologies. Vibrational spectroscopy techniques are suitable for the detection of adulteration because they allow rapid analysis, require minimal sample preparation, and can perform numerous analyses in a short time. The aim of this study was to develop rapid quantification models for the determination of melamine adulteration in a variety of SNS matrices using NIRS (near-infrared spectroscopy) in combination with multivariate data processing. Moreover, a comparison of benchtop and portable NIR instruments was carried out. Employing a stepwise approach involving OPLS-DA and PLS analysis, matrix discrimination and prediction ability were investigated. The benchtop instrument effectively discriminated among matrices (R2Y = 0.964, Q2 = 0.933), while the portable device, although showing a slightly altered pattern, maintained favorable discrimination capability (R2Y = 0.966, Q2 = 0.931). The quantitative PLS models for each SNS matrix exhibited comparable statistical indicators for both instruments with reasonable errors for melamine content estimation and prediction (RMSEE: 0.3-2.4 %, RMSEP: 0.98-2.99 %). Higher estimation and prediction errors were observed for protein-containing samples in both acquisition modes, probably due to the tendency of protein agglomeration and adhesion to different surfaces, which affects the homogeneity of the powder. Despite data loss due to the narrower spectral range and lower resolution of the portable instrument, all models were found to be suitable for predicting melamine content in sport nutrition supplements.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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