| Autor: |
Kus F; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland.; Laboratory of Protein Biochemistry, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, Gdansk, Poland., Smolenski RT; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland., Tomczyk M; Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland. |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleosides, nucleotides & nucleic acids [Nucleosides Nucleotides Nucleic Acids] 2024; Vol. 43 (8), pp. 751-760. Date of Electronic Publication: 2024 May 14. |
| DOI: |
10.1080/15257770.2024.2348742 |
| Abstrakt: |
Endothelial cells (ECs) are the first line that comes into contact with blood pathogens, pathogen-derived molecules, and factors that stimulate coagulation and inflammation. Inorganic polyphosphate (polyP) - a polymer of orthophosphate units synthesized by bacteria under stress and released by platelets upon their activation is among these factors. Bacterial and platelet polyPs differ in length, and both variants elicit different effects in eukaryotes. This study aimed to investigate how bacterial-like long-chain polyP (Lc-polyP) and platelet-like short-chain polyP (Sc-polyP) affect the functionality of cultured endothelial cells. Murine immortalized heart endothelial cells (H5V) were exposed to polyP of different chain lengths to assess the effects of these stimuli on intracellular energetics, permeability, and endothelial adhesion. We observed varying effects between Lc-polyP and Sc-polyP treatments. Lc-polyP more potently disturbs the intracellular ATP pool, a parameter strongly connected with vascular injury, whereas Sc-polyP robustly stimulates cellular adhesion to the endothelium. Both polymers similarly enhance endothelial permeability, suggesting potent immunomodulatory properties. This study provides evidence that polyP elicits profound cellular responses in endothelium depending on the polymer's length. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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