| Autor: |
Riaz M; Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan., Chang SC; Department of Emergency Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan., Tasawar Z; Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan., Sajid M; Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan., Nasreen N; Department of Botany & Zoology, Bacha Khan University, Charsadda, Pakistan., Amaro-Estrada I; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP. Carretera Federal Cuernavaca- Cuautla No. 8534,Progreso, Jiutepec, Morelos, México., Cossío-Bayúgar R; Departamento de Artropodología, Centro Nacional de Investigaciones Disciplinarias en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), Boulevard Cuauhnahuac No. 8534,Jiutepec, Morelos, Mexico., de la Fuente J; SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo 12,Ciudad Real, Spain.; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, USA., Alanazi AD; Department of Biological Science, Faculty of Sciences and Humanities, Shaqra University 11911Ad-Dawadimi 1040,Saudi Arabia., Chen CC; Department of Pathology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan.; Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan.; Ph.D. Program in Translational Medicine, Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan.; Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan., Khan A; Department of Botany and Zoology, Bacha Khan University, Charsadda, Khyber Pakhtunkhwa, Pakistan.; Department of Biology, Mountain Allison University, Sackville, New Brunswick Canada., Ben Said M; Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, Tunisia.; Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Manouba, Tunisia. |
| Abstrakt: |
Background: Theileria spp. are responsible for ovine and caprine theileriosis, leading to significant morbidity and mortality in small ruminants. The present study aims to investigate Theileria spp. infections in small ruminants from Southern Punjab in Pakistan, and genetic characterize revealed Theileria spp. isolates. Methods: A total of 93 sheep and 107 goats were sampled between May and August 2022. Blood smears were examined microscopically, and PCR amplification targeting the 18S rRNA gene was performed to detect Theileria spp. Additionally, specific PCR assays targeting 18S rRNA and ms1 partial sequences were used to identify Theileria ovis and T. lestoquardi , respectively. Results: The prevalence of Theileria spp. was significantly higher using PCR (13.5%) compared to microscopic screening (5%). Sheep showed a higher prevalence rate (19.4%) compared to goats (8.4%) ( p = 0.024). Young sheep aged ≤ 1 year were more commonly infected with Theileria spp. (41%) compared to older sheep ( p = 0.006). The prevalence of Theileria spp. was higher in sheep-only herds (37.3%) compared to goat-only herds (18%) or mixed-species herds (8.1%) ( p = 0.015). The prevalence rates of T. ovis and T. lestoquardi were 9% and 2.5%, respectively, with four animals (2 goats and 2 sheep) showing co-infection. Phylogenetic analysis revealed that our T. ovis 18S rRNA sequence clustered with previously reported sequences from sheep in Turkey, China, Spain, and goats in Tanzania. The obtained T. lestoquardi ms1 partial sequence formed a distinct cluster from other T. lestoquardi isolates in Pakistan and neighboring countries. Conclusion: Theileria spp. co-circulation in Pakistani small ruminants, particularly the presence of T. ovis and T. lestoquardi , highlights the need for attention from animal health decision-makers due to their financial and health impacts. |
