Biodegradation and antimicrobial capability-induced heavy metal resistance of the marine-derived actinomycetes Nocardia harenae JJB5 and Amycolatopsis marina JJB11.

Autor: El-Sayed MH; Department of Biology, College of Science and Arts-Rafha, Northern Border University, Arar, Saudi Arabia. Mohammed.Ahmed@nbu.edu.sa.; Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt. Mohammed.Ahmed@nbu.edu.sa., Abdellatif MM; Department of Biology, College of Science and Arts-Rafha, Northern Border University, Arar, Saudi Arabia., Mostafa HM; Department of Chemistry, College of Science and Arts-Rafha, Northern Border University, Arar, Saudi Arabia., Elsehemy IA; Department of Natural and Microbial Products Chemistry, Division of Pharmaceutical and Drug Industries Research, National Research Centre, Cairo, Egypt., Kobisi AEA; Plant Protection Department, Desert Research Center, Cairo, Egypt.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 May 14; Vol. 40 (7), pp. 202. Date of Electronic Publication: 2024 May 14.
DOI: 10.1007/s11274-024-04006-x
Abstrakt: Currently, heavy metal-resistant (HMR) marine actinomycetes have attracted much attention worldwide due to their unique capabilities. In this study, 27 marine-derived actinomycetes were isolated from coastal beaches in the Arabian Gulf of Al-Jubail in Saudi Arabia and screened for resistance to 100 mg/L of the heavy metals Cd 2+ , Cr 6+ , Cu 2+ , Fe 2+ , Pb 2+ , and Ni 2+ using different assay techniques. Six isolates were selected as HMRs, of which two isolates, JJB5 and JJB11, exhibited the highest maximum tolerance concentrations (200- > 300 mg/L). Both isolates were the highest among six-HMR screened for their biodegradation potential of plastics low-density polyethylene, polystyrene, and polyvinyl chloride, recording the highest weight loss (15 ± 1.22 - 65 ± 1.2%) in their thin films. They also showed the highest biodegradability of the pesticides acetamiprid, chlordane, hexachlorocyclohexane, indoxacarb and lindane, indicating promising removal capacities (95.70-100%) for acetamiprid and indoxacarb using HPLC analysis. Additionally, the cell-free filtrate (CFF) of both isolates displayed the highest antimicrobial activity among the six-HMR screened against a variety of microbial test strains, recording the highest inhibition zone diameters (13.76 ± 0.66 - 26.0 ± 1.13 mm). GC‒MS analyses of the ethyl acetate extract of their CFFs revealed the presence of diverse chemical compounds with a multitude of remarkable biological activities. Based on their spore morphology and wall-chemotype, they were assigned to the nocardioform-actinomycetes. Furthermore, their phenotypic characteristics, together with 16S rRNA gene sequencing (OR121525-OR121526), revealed them as Nocardia harenae JJB5 and Amycolatopsis marina JJB11. Our results suggest that marine HMR actinomycetes are promising candidates for various biotechnological applications.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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