Antimicrobial efficacy of direct air gas soft jet plasma for the in vitro reduction of oral bacterial biofilms.

Autor: Puca V; Department of Pharmacy, University 'G. d'Annunzio' of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy., Marinacci B; Department of Pharmacy, University 'G. d'Annunzio' of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Pinti M; Department of Pharmacy, University 'G. d'Annunzio' of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy., Di Cintio F; Department of Oral, Medical and Biotechnological Sciences, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy.; Center for Advanced Studies and Technology (CAST), University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Sinjari B; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Di Marcantonio MC; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Mincione G; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Acharya TR; Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South Korea., Kaushik NK; Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South Korea., Choi EH; Plasma Bioscience Research Center, Department of Electrical and Biological Physics, Kwangwoon University, Seoul, 01897, South Korea., Sallese M; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy.; Center for Advanced Studies and Technology (CAST), University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Guarnieri S; Department of Neuroscience, Imaging and Clinical Sciences, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy.; Center for Advanced Studies and Technology (CAST), University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy., Grande R; Department of Pharmacy, University 'G. d'Annunzio' of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy. rossella.grande@unich.it.; Center for Advanced Studies and Technology (CAST), University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy. rossella.grande@unich.it., Perrotti V; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' of Chieti-Pescara, Chieti, Italy. v.perrotti@unich.it.; UdA-TechLab, Research Center, University 'G. d'Annunzio' of Chieti-Pescara, 66100, Chieti, Italy. v.perrotti@unich.it.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 13; Vol. 14 (1), pp. 10882. Date of Electronic Publication: 2024 May 13.
DOI: 10.1038/s41598-024-61438-z
Abstrakt: The aim of this study was to evaluate the antimicrobial efficacy of an air gas soft jet CAP for its potential use in removing oral biofilms, given that plasma-based technologies have emerged as promising methods in periodontology. Two types of biofilms were developed, one by Streptococcus mutans UA 159 bacterial strain and the other by a complex mixture of saliva microorganisms isolated from a patient with periodontitis. This latter biofilm was characterized via Next Generation Sequencing to determine the main bacterial phyla. The CAP source was applied at a distance of 6 mm for different time points. A statistically significant reduction of both CFU count and XTT was already detected after 60 s of CAP treatment. CLSM analysis supported CAP effectiveness in killing the microorganisms inside the biofilm and in reducing the thickness of the biofilm matrix. Cytotoxicity tests demonstrated the possible use of CAP without important side effects towards human gingival fibroblasts cell line. The current study showed that CAP treatment was able to significantly reduce preformed biofilms developed by both S. mutans and microorganisms isolated by a saliva sample. Further studies should be conducted on biofilms developed by additional saliva donors to support the potential of this innovative strategy to counteract oral pathogens responsible for periodontal diseases.
(© 2024. The Author(s).)
Databáze: MEDLINE
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