Immunoadsorption as a method of antibody donation during the COVID-19 pandemic.

Autor: Rothenburg J; Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany.; Cytiva, Dreieich, Germany., Rink-Baron S; Miltenyi Biotec, Bergisch Gladbach, Germany., Müller L; Institute of Virology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Ostermann PN; Institute of Virology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Fischer JC; Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Hermsen D; Central Institute of Laboratory Medicine, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Stegbauer J; Department of Nephrology, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Moldenhauer A; Institute for Transplant Diagnostics and Cell Therapeutics, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany.; Saarland University, Homburg, Germany.
Jazyk: angličtina
Zdroj: Vox sanguinis [Vox Sang] 2024 Aug; Vol. 119 (8), pp. 792-800. Date of Electronic Publication: 2024 May 13.
DOI: 10.1111/vox.13647
Abstrakt: Background and Objectives: Initial therapeutic efforts to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) included the use of plasma from convalescent donors containing anti-SARS-CoV-2 antibodies. High-neutralizing antibody titres are required for therapeutic efficacy. This study aims to show that immunoadsorption followed by tangential flow filtration can be used to obtain antibody concentrates with high-neutralizing capacities.
Materials and Methods: Eligible donors (n = 10, five males and three females) underwent immunoadsorption using adsorber columns specific for human antibodies. Glycine-washed out eluates of 1.5 L volume were further concentrated by tangential flow filtration using 30 kDa ultrafiltration membranes. The same membranes were applied for diafiltrations to exchange residual glycine for 0.9% normal saline.
Results: Antibody concentrates were obtained within 8 h from the start of donation and had 4.58 ± 1.95, 3.28 ± 1.28 and 2.02 ± 0.92 times higher total IgG, IgA and IgM concentrations, 3.29 ± 1.62 and 3.74 ± 0.6 times higher SARS-CoV-2 N and S antibody concentrations and 3.85 ± 1.71 times higher SARS-CoV-2 S-specific IgG concentrations compared to the donors' peripheral blood. The specific SARS-CoV-2 virus neutralization capacities increased in all but one concentrate. All antibody concentrates (50-70 mL final volume) passed microbiological tests, were free of hazardous glycine levels and could be stored at -80°C and 4°C for 1 year with 20 ± 3% antibody loss.
Conclusion: Immunoadsorption followed by tangential flow filtration is a feasible procedure to collect IgG, IgA and IgM as well as SARS-CoV-2 N- and S-specific antibody concentrates of low volume, free of albumin and coagulation factors. Whether these concentrates can be used as passive immunisation in infected patients remains to be elucidated.
(© 2024 The Authors. Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion.)
Databáze: MEDLINE
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