The clinical significance of long non-coding RNAs MALAT1 and CASC2 in the diagnosis of HCV-related hepatocellular carcinoma.

Autor: Golam RM; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Khalil MAF; Department of Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt., Shaker OG; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt., Ahmed TI; Department of Internal Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Elguaad MKA; Department of Medical Physiology, Faculty of Medicine, Fayoum University, Fayoum, Egypt., Hassan EA; Department of Tropical Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt., El-Ansary MRM; Department of Medical Microbiology and Immunology, Faculty of Medicine, Misr University for Science and Technology (MUST), Giza, Egypt., Ismail A; Biochemistry and Molecular Biology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt., Kandil YI; Biochemistry and Molecular Biology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt.; Department of Biochemistry, Faculty of Pharmacy, Sinai University-Kantara Branch, Ismailia, Egypt., Mohammed OA; Department of Pharmacology, College of Medicine, University of Bisha, Bisha, Saudi Arabia., Doghish AS; Department of Biochemistry, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo, Egypt.; Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, Egypt.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 May 13; Vol. 19 (5), pp. e0303314. Date of Electronic Publication: 2024 May 13 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0303314
Abstrakt: Background: Globally, hepatocellular carcinoma (HCC) is the second most common cause of cancer-related death due to a lack of early predictive and/or diagnostic tools. Thus, research for a new biomarker is important. LncRNAs play a functional role in target gene regulation and their deregulation is associated with several pathological conditions including HCC.
Objective: This study aimed to explore the diagnostic potential of two LncRNAs MALAT1 and CASC2 in HCC compared to the routinely used diagnostic biomarker.
Materials and Methods: The current study is a case-control study carried out at Fayoum University Hospital and conducted on 89 individuals. The study included three groups of 36 HCC patients on top of HCV(HCC/HCV), 33 HCV patients, and 20 healthy volunteers as a control group. All study subjects were subjected to radiological examinations. The determination of CBC was performed by the automated counter and liver function tests by the enzymatic method were performed. In addition, HCV RNA quantification and the expression level of two LncRNAs (MALAT1 and CASC2) were performed by qRT-PCR.
Results: The results revealed a statistically significant difference between study groups regarding liver function tests with a higher mean in HCC/HCV group. Also, serum MALAT1 significantly up-regulated in HCV (11.2±2.8) and HCC/HCV (4.56±1.4) compared to the control group. Besides, serum CASC2 levels in the HCV group were significantly upregulated (14.9±3.6), while, downregulated in the HCC group (0.16± 0.03). Furthermore, The ROC analysis for diagnostic efficacy parameters indicated that CASC2 has higher accuracy (94.6%) and sensitivity (97.2%) for HCC diagnosis than AFP with an accuracy of (90.9%), sensitivity (69.4%), and MALAT1 showed an accuracy of (56.9%), sensitivity (72.2%).
Conclusion: Our study results indicated that CASC2 is a promising biomarker and is considered better and could help in HCC diagnosis on top of HCV than MALAT1 and the routine biomarker AFP.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Golam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje