The effects of probiotic-based additives on aflatoxin intoxication in Piaractus mesopotamicus: a study of liver histology and metabolic performance.

Autor: Godoy AC; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil. ufgd.work.group.aqua@gmail.com., Ziemniczak HM; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil., Fantini-Hoag L; School of Fisheries, Aquaculture and Aquatic Science, Auburn University, 203 Swingle Hall, 36849, Auburn, AL, United States of America., da Silva WV; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil., Ferreira ACV; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil., Saturnino KC; Instituto de Desenvolvimento Agrário e Regional Quadra Sete (Fl.31), Universidade Federal do Sul e Sudeste Do Pará, Rua Nova Marabá, 68507590, Marabá, Pará, Brazil., Neu DH; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil., Gandra JR; Instituto de Desenvolvimento Agrário, Universidade Federal de Jataí, BR 364 km 195, Setor Parque Industrial nº 3800, 75801615, Jataí, Goiás, Brazil., de Padua Pereira U; Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid 445 Km, 86057970, Londrina, Paraná, Brazil., Honorato CA; Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rodovia Dourados - Itahum, km 12, 79804970, Dourados, Mato Grosso do Sul, Brazil.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Aug; Vol. 48 (4), pp. 2281-2294. Date of Electronic Publication: 2024 May 13.
DOI: 10.1007/s11259-024-10409-w
Abstrakt: Mycotoxins, produced by fungi, can contaminate fish food and harm their health. Probiotics enhance immune balance and primarily function in the animal intestine. This study aimed to assess aflatoxin's impact on Piaractus mesopotamicus and explore probiotic-based additive (PBA) benefits in mitigating these effects, focusing on antioxidant activity, biochemical indices, and hepatic histopathology. Two experiments were conducted using P. mesopotamicus fry. The first experimental assay tested various levels of aflatoxin B1 (0.0, 25.0, 50.0, 100.0, 200.0, and 400.0 µg kg -1 ) over a 10-day period. The second experimental assay examined the efficacy of the probiotic (supplemented at 0.20%) in diets with different levels of aflatoxin B1 (0.0, 25.0, and 400.0 µg kg -1 ) for 15 days. At the end of each assay, the fish underwent a 24-hour fasting period, and the survival rate was recorded. Six liver specimens from each treatment group were randomly selected for metabolic indicator assays, including superoxide dismutase, catalase, alanine aminotransferase, aspartate aminotransferase, and albumin. Additionally, histopathological analysis was performed on six specimens. The initial study discovered that inclusion rates above 25.0 µg kg -1 resulted in decreased activity of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALB (albumin), CAT (catalase), and SOD (superoxide dismutase), accompanied by liver histopathological lesions. In the second study, the inclusion of PBA in diets contaminated with AFB1 improved the activity of AST and ALT up to 25.0 µg kg -1 of AFB1, with no histopathological lesions observed. The study demonstrated the hepatoprotective effects of PBA in diets contaminated with AFB1. The enzyme activity and hepatic histopathology were maintained, indicating a reduction in damage caused by high concentrations of AFB1 (400.0 µg kg -1 of AFB1). The adverse effects of AFB1 on biochemical and histopathological parameters were observed from 25.0 µg kg -1 onwards. Notably, PBA supplementation enhanced enzymatic activity at a concentration of 25 µg kg -1 of AFB1 and mitigated the effects at 400.0 µg kg -1 of AFB1. The use of PBAs in pacu diets is highly recommended as they effectively neutralize the toxic effects of AFB1 when added to diets containing 25.0 µg kg -1 AFB1. Dietary inclusion of aflatoxin B1 at a concentration of 25.0 µg kg -1 adversely affects the liver of Piaractus mesopotamicus (Pacu). However, the addition of a probiotic-based additive (PBA) to the diets containing this concentration of aflatoxin neutralized its toxic effects. Therefore, the study recommends the use of PBAs in Pacu diets to mitigate the adverse effects of aflatoxin contamination.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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