Inorganic Biomaterials Shape the Transcriptome Profile to Induce Endochondral Differentiation.

Autor: Murali A; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Brokesh AM; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Cross LM; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Kersey AL; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Jaiswal MK; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Singh I; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Department of Cell Biology and Genetics, College of Medicine, Texas A&M University, Bryan, TX, 77807-3260, USA.; Interdisciplinary Program in Genetics and Genomics, Texas A&M University, College Station, TX, 77843, USA., Gaharwar A; Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Interdisciplinary Program in Genetics and Genomics, Texas A&M University, College Station, TX, 77843, USA.; Department of Material Science and Engineering, College of Engineering, Texas A&M University, College Station, TX, 77843, USA.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Aug; Vol. 11 (29), pp. e2402468. Date of Electronic Publication: 2024 May 13.
DOI: 10.1002/advs.202402468
Abstrakt: Minerals play a vital role, working synergistically with enzymes and other cofactors to regulate physiological functions including tissue healing and regeneration. The bioactive characteristics of mineral-based nanomaterials can be harnessed to facilitate in situ tissue regeneration by attracting endogenous progenitor and stem cells and subsequently directing tissue-specific differentiation. Here, cellular responses of human mesenchymal stem/stromal cells to traditional bioactive mineral-based nanomaterials, such as hydroxyapatite, whitlockite, silicon-dioxide, and the emerging synthetic 2D nanosilicates are investigated. Transcriptome sequencing is utilized to probe the cellular response and determine the significantly affected signaling pathways due to exposure to these inorganic nanomaterials. Transcriptome profiles of stem cells treated with nanosilicates reveals a stabilized skeletal progenitor state suggestive of endochondral differentiation. This observation is bolstered by enhanced deposition of matrix mineralization in nanosilicate treated stem cells compared to control or other treatments. Specifically, use of 2D nanosilicates directs osteogenic differentiation of stem cells via activation of bone morphogenetic proteins and hypoxia-inducible factor 1-alpha signaling pathway. This study provides  insight into impact of nanomaterials on cellular gene expression profile and predicts downstream effects of nanomaterial induction of endochondral differentiation.
(© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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