Autor: |
Colombo RP; Laboratorio de microbiología del Suelo, Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina., Silvani VA; Laboratorio de microbiología del Suelo, Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina., Benavidez ME; Laboratorio de microbiología del Suelo, Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina., Scotti A; Laboratorio Bioambiental, Comisión Nacional de Energía Atómica, International Center of Earth Science, San Rafael, Mendoza, Argentina., Godeas AM; Laboratorio de microbiología del Suelo, Instituto de Biodiversidad y Biología Experimental y Aplicada, CONICET-UBA, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. |
Abstrakt: |
Arbuscular mycorrhizal fungi (AMF) have different biological mechanisms to alleviate stressful conditions in heavy metals (HMs) polluted soil. These mechanisms were widely assessed under controlled/greenhouse conditions, but scarcely studied at pilot or territory scale. The aim of this study was to evaluate the response of two Rhizophagus intraradices strains isolated from soils with different histories of pollution, in association with Senecio bonariensis plants, growing in an engineering vegetal depuration module filled with artificially HMs polluted substrate. Plants inoculated with GC3 strain uptook low amounts of HMs and translocated them to shoot biomass. Heavy metals (Mg, Zn, Mn, Cr, Cu and Ni) and macronutrients (Ca, K, S and P) were accumulated in roots of S. bonariensis when inoculated with GB8 strain, limiting their translocation to the shoot. Uninoculated plants showed high translocation of all studied elements to shoot tissues. Concluding, tested R. intraradices strains have exhibited different phytoprotection mechanisms under extremely toxic concentrations of HMs. Moreover, the development of the assay at such a high Technological Readiness Level represents a novel contribution in this field of study. |