Evaluating the Impact of Vitamin D 3 on NF-κB and JAK/STAT Signaling Pathways in Drosophila melanogaster .

Autor: Hardiyanti W; Postgraduate Program in Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Djabir YY; Department of Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Fatiah D; Postgraduate Program in Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Pratama MR; Postgraduate Program in Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Putri TZAD; Postgraduate Program in Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Chaeratunnisa R; Undergraduate Program in Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Latada NP; Unhas Fly Research Group, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Mudjahid M; Department of Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Asri RM; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia., Nainu F; Department of Pharmacy, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia.; Unhas Fly Research Group, Faculty of Pharmacy, Hasanuddin University, Tamalanrea, Makassar 90245, Indonesia.
Jazyk: angličtina
Zdroj: ACS omega [ACS Omega] 2024 Apr 25; Vol. 9 (18), pp. 20135-20141. Date of Electronic Publication: 2024 Apr 25 (Print Publication: 2024).
DOI: 10.1021/acsomega.4c00134
Abstrakt: This study delved into the consequences of prolonged administration of vitamin D 3 on innate immune systems, particularly NF-κB and JAK/STAT, in Drosophila melanogaster . The outcomes indicated that vitamin D 3 treatment exhibited a notable capacity to improve the survival of adult flies with compromised immune functions, a condition induced by the loss of PGRP-LB, particularly when the flies were exposed to heat-killed Escherichia coli . The PGRP-LB Δ mutant line that was treated with heat-killed E. coli experienced reduced survival. Treatment of heat-killed E. coli -treated PGRP-LB Δ with vitamin D 3 resulted in improved survival, and this phenotypic feature might be due to the downregulation of gene expression in the NF-κB and JAK/STAT pathways. However, a higher concentration of vitamin D 3 was associated with decreased survival, potentially linked to intricate immunological responses. The research also underscored the influence of vitamin D 3 on the expression of antioxidant genes, sod1 and sod2 , indicating an augmented resistance to oxidative stress. Further, this study revealed the effect of vitamin D 3 on the reproductive status of the autoinflammatory model, showing an increase in pupae and adult flies with a treatment of 10 mM vitamin D 3 , suggesting the potential benefits of vitamin D 3 on the reproductive profile. Overall, this study provides preliminary insights into the complex interactions between vitamin D 3 , immune pathways, oxidative responses in the cell, and reproduction in Drosophila .
Competing Interests: The authors declare no competing financial interest.
(© 2024 The Authors. Published by American Chemical Society.)
Databáze: MEDLINE
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