The gut microbiome, resistome, and mycobiome in preterm newborn infants and mouse pups: lack of lasting effects by antimicrobial therapy or probiotic prophylaxis.

Autor: Yuu EY; Data Analytics & Computational Statistics, Hasso Plattner Institute, University of Potsdam, Prof.-Dr.-Helmert-Straße 2-3, 14482 , Potsdam, Germany., Bührer C; Charité - Universitätsmedizin, Berlin, Germany., Eckmanns T; Robert Koch Institute, Berlin, Germany., Fulde M; Department of Mathematics and Computer Science, Freie Universität Berlin, 14195, Berlin, Germany., Herz M; Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany., Kurzai O; Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany.; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11A, 07745 , Jena, Germany., Lindstedt C; Charité - Universitätsmedizin, Berlin, Germany., Panagiotou G; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11A, 07745 , Jena, Germany.; Faculty of Biological Sciences, Friedrich Schiller University, 07745, Jena, Germany.; Department of Medicine, The University of Hong Kong, Hong Kong, China., Piro VC; Data Analytics & Computational Statistics, Hasso Plattner Institute, University of Potsdam, Prof.-Dr.-Helmert-Straße 2-3, 14482 , Potsdam, Germany.; Department of Mathematics and Computer Science, Freie Universität Berlin, 14195, Berlin, Germany., Radonic A; Robert Koch Institute, Berlin, Germany., Renard BY; Data Analytics & Computational Statistics, Hasso Plattner Institute, University of Potsdam, Prof.-Dr.-Helmert-Straße 2-3, 14482 , Potsdam, Germany., Reuss A; Robert Koch Institute, Berlin, Germany.; Ministry of Justice and Health, Schleswig-Holstein, Kiel , Germany., Siliceo SL; Department of Microbiome Dynamics, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11A, 07745 , Jena, Germany., Thielemann N; Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany., Thürmer A; Robert Koch Institute, Berlin, Germany., Vorst KV; Department of Mathematics and Computer Science, Freie Universität Berlin, 14195, Berlin, Germany., Wieler LH; Data Analytics & Computational Statistics, Hasso Plattner Institute, University of Potsdam, Prof.-Dr.-Helmert-Straße 2-3, 14482 , Potsdam, Germany.; Robert Koch Institute, Berlin, Germany., Haller S; Robert Koch Institute, Berlin, Germany. HallerS@rki.de.
Jazyk: angličtina
Zdroj: Gut pathogens [Gut Pathog] 2024 May 12; Vol. 16 (1), pp. 27. Date of Electronic Publication: 2024 May 12.
DOI: 10.1186/s13099-024-00616-w
Abstrakt: Background: Enhancing our understanding of the underlying influences of medical interventions on the microbiome, resistome and mycobiome of preterm born infants holds significant potential for advancing infection prevention and treatment strategies. We conducted a prospective quasi-intervention study to better understand how antibiotics, and probiotics, and other medical factors influence the gut development of preterm infants. A controlled neonatal mice model was conducted in parallel, designed to closely reflect and predict exposures. Preterm infants and neonatal mice were stratified into four groups: antibiotics only, probiotics only, antibiotics followed by probiotics, and none of these interventions. Stool samples from both preterm infants and neonatal mice were collected at varying time points and analyzed by 16 S rRNA amplicon sequencing, ITS amplicon sequencing and whole genome shotgun sequencing.
Results: The human infant microbiomes showed an unexpectedly high degree of heterogeneity. Little impact from medical exposure (antibiotics/probiotics) was observed on the strain patterns, however, Bifidobacterium bifidum was found more abundant after exposure to probiotics, regardless of prior antibiotic administration. Twenty-seven antibiotic resistant genes were identified in the resistome. High intra-variability was evident within the different treatment groups. Lastly, we found significant effects of antibiotics and probiotics on the mycobiome but not on the microbiome and resistome of preterm infants.
Conclusions: Although our analyses showed transient effects, these results provide positive motivation to continue the research on the effects of medical interventions on the microbiome, resistome and mycobiome of preterm infants.
(© 2024. The Author(s).)
Databáze: MEDLINE
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