Deposition of collagen III and alterations in basement membrane integrity as candidate prognostic markers in prostate cancer.

Autor: Pinheiro LCL; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Pupim ACE; Laboratory of Enteric Neuroscience, Department of Histology, Londrina State University, Londrina, PR, Brazil., Pereira ÉR; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Ahrens TM; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Mendonça AC; Laboratory of Extracellular Matrix, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Francelino AL; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Araújo EJA; Laboratory of Enteric Neuroscience, Department of Histology, Londrina State University, Londrina, PR, Brazil., Guembarovski AFML; Micropar - Laboratory of Pathological Anatomy and Cytology, Londrina, PR, Brazil., Fuganti PE; Cancer Hospital of Londrina - HCL, Londrina, PR, Brazil., Vanzela ALL; Laboratory of Cytogenetics and Plant Diversity, Londrina, PR, Brazil., Colus IMS; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Favaron PO; Laboratory of Extracellular Matrix, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Miqueloto CA; Laboratory of Extracellular Matrix, Department of General Biology, Londrina State University, Londrina, PR, Brazil., Guembarovski RL; Laboratory of Mutagenesis and Oncogenetics, Department of General Biology, Londrina State University, Londrina, PR, Brazil. Electronic address: robertalosi@uel.br.
Jazyk: angličtina
Zdroj: Experimental cell research [Exp Cell Res] 2024 Jun 01; Vol. 439 (1), pp. 114077. Date of Electronic Publication: 2024 May 11.
DOI: 10.1016/j.yexcr.2024.114077
Abstrakt: The extracellular matrix surrounding the tumor undergoes changes in its organization during the metastasis process. The present study aims to quantify total collagen, collagen I (Col I) and collagen III (Col III), analyze the alignment of collagen fibers and assess the basement membrane integrity in samples from patients with metastatic and non-metastatic prostate cancer. Tissue samples from 60 patients were classified into groups based on prognostic parameters: better prognosis (n = 20), worse prognosis without metastasis (n = 23) and metastatic (n = 17). Picrosirius red with further analysis under polarizing microscope was used to quantify (with validation using immunohistochemistry) and analyze collagen alignment, and Periodic Acid Schiff staining was used to analyze the basement membrane integrity. The Col I/Col III ratio was found to be higher in the metastatic group than in the groups with better prognosis (p = 0.012) and worse prognosis without metastasis (p = 0.018). Basement membrane integrity constitution in malignant tumor tissue differed from that of adjacent non-tumor tissue (p < 0.001). Moreover, the worsening in the tumor tissue integrity was positively correlated with worse prognostic parameters. All in all, absence of Col III and basement membrane integrity might be indicators of poor prognosis in prostate cancer.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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