A reproducible and affordable method of conducting luciferase assay.

Autor: Reddy K; Department of Molecular Oncology, Cancer Institute WIA, Adyar, Chennai, 600 036, India., Ramachandran B; Department of Molecular Oncology, Cancer Institute WIA, Adyar, Chennai, 600 036, India. Electronic address: balajiphd@gmail.com.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2024 Sep; Vol. 692, pp. 115558. Date of Electronic Publication: 2024 May 10.
DOI: 10.1016/j.ab.2024.115558
Abstrakt: Commercially available glow luciferase assay kits are widely popular and convenient to use. However, concerning high-throughput screening, commercial kits are limited by huge running costs. As an alternative to commercial luciferase assay kits, this study presents a cost-effective and efficient methodology of performing a simple and rapid laboratory flash luciferase assay. The proposed luciferase assay method has a versatile use ranging from screening lysates in a microplate reader for quantitative assay as well as screening live cells qualitatively or quantitatively under an imaging system.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: