Mesenchymal Stem Cells from the Deciduous Tooth Pulp Lose their Ability to Suppress the Differentiation of Dendritic Cells during Long-Term Culturing.

Autor: Lupatov AY; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. alupatov@mail.ru., Vakhrushev IV; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia., Saryglar RY; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia., Yarygin KN; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 Mar; Vol. 176 (5), pp. 672-679. Date of Electronic Publication: 2024 May 11.
DOI: 10.1007/s10517-024-06089-w
Abstrakt: A culture of cells expressing markers of mesenchymal stem cells (MSC) (CD73, CD90, CD44, CD29, and CD49b), but not hematopoietic cell markers, and capable of multilineage differentiation was isolated from the deciduous tooth pulp. Co-culturing with immature dendritic cells in the presence of LPS did not reveal an ability of the MSC to suppress the maturation of dendritic cells. On the contrary, co-culturing of MSC with monocytes in the presence of granulocyte-macrophage CSF and IL-4 led to complete suppression of monocyte differentiation into dendritic cells. However, long-term culturing of MSC from dental pulp showed that by the passage 11, they almost completely lose their suppressor ability. These results indicate that the immunological properties of MSC can change during culturing without changing their phenotypic markers. This should be taken into account when creating biomedical cell products.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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