| Autor: |
Huang M; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Liu J; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Yuan Z; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Xu Y; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Guo Y; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Yang S; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China., Fei H; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. |
| Abstrakt: |
C-type lectins in organisms play an important role in the process of innate immunity. In this study, a C-type lectin belonging to the DC-SIGN class of Micropterus salmoides was identified. MsDC-SIGN is classified as a type II transmembrane protein. The extracellular segment of MsDC-SIGN possesses a coiled-coil region and a carbohydrate recognition domain (CRD). The key amino acid motifs of the extracellular CRD of MsDC-SIGN in Ca 2+ -binding site 2 were EPN (Glu-Pro-Asn) and WYD (Trp-Tyr-Asp). MsDC-SIGN-CRD can bind to four pathogen-associated molecular patterns (PAMPs), including lipopolysaccharide (LPS), glucan, peptidoglycan (PGN), and mannan. Moreover, it can also bind to Gram-positive, Gram-negative bacteria, and fungi. Its CRD can agglutinate microbes and displays D-mannose and D-galactose binding specificity. MsDC-SIGN was distributed in seven tissues of the largemouth bass, among which the highest expression was observed in the liver, followed by the spleen and intestine. Additionally, MsDC-SIGN was present on the membrane of M . salmoides leukocytes, thereby augmenting the phagocytic activity against bacteria. In a subsequent investigation, the expression patterns of the MsDC-SIGN gene and key genes associated with the TLR signaling pathway (TLR4, NF-κB, and IL10) exhibited an up-regulated expression response to the stimulation of Aeromonas hydrophila . Furthermore, through RNA interference of MsDC-SIGN, the expression level of the DC-SIGN signaling pathway-related gene (RAF1) and key genes associated with the TLR signaling pathway (TLR4, NF-κB, and IL10) was decreased. Therefore, MsDC-SIGN plays a pivotal role in the immune defense against A . hydrophila by modulating the TLR signaling pathway. |
