| Autor: |
Zaszczyńska A; Laboratory of Polymers Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5B, 02-106 Warsaw, Poland., Gradys A; Laboratory of Polymers Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5B, 02-106 Warsaw, Poland., Ziemiecka A; Laboratory of Cell Research and Application, 02-106 Warsaw, Poland., Szewczyk PK; Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, 30-059 Krakow, Poland., Tymkiewicz R; Laboratory of Polymers Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5B, 02-106 Warsaw, Poland., Lewandowska-Szumieł M; Laboratory of Cell Research and Application, 02-106 Warsaw, Poland.; Department of Histology and Embryology, Centre for Biostructure Research, Medical University of Warsaw, Centre for Preclinical Research and Technology, 02-106 Warsaw, Poland., Stachewicz U; Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, 30-059 Krakow, Poland., Sajkiewicz PŁ; Laboratory of Polymers Biomaterials, Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5B, 02-106 Warsaw, Poland. |
| Abstrakt: |
Nanofibrous materials generated through electrospinning have gained significant attention in tissue regeneration, particularly in the domain of bone reconstruction. There is high interest in designing a material resembling bone tissue, and many scientists are trying to create materials applicable to bone tissue engineering with piezoelectricity similar to bone. One of the prospective candidates is highly piezoelectric poly(vinylidene fluoride) (PVDF), which was used for fibrous scaffold formation by electrospinning. In this study, we focused on the effect of PVDF molecular weight (180,000 g/mol and 530,000 g/mol) and process parameters, such as the rotational speed of the collector, applied voltage, and solution flow rate on the properties of the final scaffold. Fourier Transform Infrared Spectroscopy allows for determining the effect of molecular weight and processing parameters on the content of the electroactive phases. It can be concluded that the higher molecular weight of the PVDF and higher collector rotational speed increase nanofibers' diameter, electroactive phase content, and piezoelectric coefficient. Various electrospinning parameters showed changes in electroactive phase content with the maximum at the applied voltage of 22 kV and flow rate of 0.8 mL/h. Moreover, the cytocompatibility of the scaffolds was confirmed in the culture of human adipose-derived stromal cells with known potential for osteogenic differentiation. Based on the results obtained, it can be concluded that PVDF scaffolds may be taken into account as a tool in bone tissue engineering and are worth further investigation. |
