| Autor: |
Hahn O; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany., Waheed TO; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany., Sridharan K; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany., Huemerlehner T; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany., Staehlke S; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany., Thürling M; Microfluidics, Faculty of Mechanical Engineering and Marine Technology, University of Rostock, 18059 Rostock, Germany., Boeckmann L; Clinic and Polyclinic for Dermatology and Venerology Rostock, Rostock University Medical Center, 18057 Rostock, Germany., Meister M; Leibniz-Institute for Plasma Science and Technology e.V., 17489 Greifswald, Germany., Masur K; Leibniz-Institute for Plasma Science and Technology e.V., 17489 Greifswald, Germany., Peters K; Institute of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany. |
| Abstrakt: |
Cold atmospheric pressure plasma (CAP) offers a variety of therapeutic possibilities and induces the formation of reactive chemical species associated with oxidative stress. Mesenchymal stem/stromal cells (MSCs) play a central role in tissue regeneration, partly because of their antioxidant properties and ability to migrate into regenerating areas. During the therapeutic application, MSCs are directly exposed to the reactive species of CAP. Therefore, the investigation of CAP-induced effects on MSCs is essential. In this study, we quantified the amount of ROS due to the CAP activation of the culture medium. In addition, cell number, metabolic activity, stress signals, and migration were analyzed after the treatment of MSCs with a CAP-activated medium. CAP-activated media induced a significant increase in ROS but did not cause cytotoxic effects on MSCs when the treatment was singular and short-term (one day). This single treatment led to increased cell migration, an essential process in wound healing. In parallel, there was an increase in various cell stress proteins, indicating an adaptation to oxidative stress. Repeated treatments with the CAP-activated medium impaired the viability of the MSCs. The results shown here provide information on the influence of treatment frequency and intensity, which could be necessary for the therapeutic application of CAP. |
