Manganese Overexposure Alters Neurogranin Expression and Causes Behavioral Deficits in Larval Zebrafish.

Autor: Alba-González A; Department of Biology, Faculty of Sciences, University of A Coruña, 15008 A Coruña, Spain.; Centro Interdisciplinar de Química y Biología, (CICA), University of A Coruña, 15071 A Coruña, Spain., Dragomir EI; Department of Cell and Developmental, University College London, London, WC1E 6BT, UK., Haghdousti G; Department of Cell and Developmental, University College London, London, WC1E 6BT, UK., Yáñez J; Department of Biology, Faculty of Sciences, University of A Coruña, 15008 A Coruña, Spain.; Centro Interdisciplinar de Química y Biología, (CICA), University of A Coruña, 15071 A Coruña, Spain., Dadswell C; School of Life Sciences, University of Sussex, Brighton, BN1 9QJ, UK., González-Méndez R; School of Life Sciences, University of Sussex, Brighton, BN1 9QJ, UK., Wilson SW; Department of Cell and Developmental, University College London, London, WC1E 6BT, UK., Tuschl K; UCL GOSH Institute of Child Health, University College London, London, WC1N 1EH, UK., Folgueira M; Department of Biology, Faculty of Sciences, University of A Coruña, 15008 A Coruña, Spain.; Centro Interdisciplinar de Química y Biología, (CICA), University of A Coruña, 15071 A Coruña, Spain.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Apr 30; Vol. 25 (9). Date of Electronic Publication: 2024 Apr 30.
DOI: 10.3390/ijms25094933
Abstrakt: Manganese (Mn), a cofactor for various enzyme classes, is an essential trace metal for all organisms. However, overexposure to Mn causes neurotoxicity. Here, we evaluated the effects of exposure to Mn chloride (MnCl 2 ) on viability, morphology, synapse function (based on neurogranin expression) and behavior of zebrafish larvae. MnCl 2 exposure from 2.5 h post fertilization led to reduced survival (60%) at 5 days post fertilization. Phenotypical changes affected body length, eye and olfactory organ size, and visual background adaptation. This was accompanied by a decrease in both the fluorescence intensity of neurogranin immunostaining and expression levels of the neurogranin-encoding genes nrgna and nrgnb , suggesting the presence of synaptic alterations. Furthermore, overexposure to MnCl 2 resulted in larvae exhibiting postural defects, reduction in motor activity and impaired preference for light environments. Following the removal of MnCl 2 from the fish water, zebrafish larvae recovered their pigmentation pattern and normalized their locomotor behavior, indicating that some aspects of Mn neurotoxicity are reversible. In summary, our results demonstrate that Mn overexposure leads to pronounced morphological alterations, changes in neurogranin expression and behavioral impairments in zebrafish larvae.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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