Light-Emitting-Diode-Induced Fluorescence from Organic Dyes for Application in Excitation-Emission Fluorescence Spectroscopy for Food System Analysis.

Autor:	Vladev V; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.; Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sankt Peterburg Blvd., 4002 Plovdiv, Bulgaria., Brazkova M; Department of Biotechnology, Technological Faculty, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria., Bozhkov S; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria., Angelova G; Department of Biotechnology, Technological Faculty, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria., Blazheva D; Department of Microbiology, Technological Faculty, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria., Minkova S; Department of Physics and Biophysics, Medical University-Varna, 84 Tzar Osvoboditel Blvd., 9000 Varna, Bulgaria., Nikolova K; Department of Mathematics, Physics and Information Technologies, Faculty of Economics, University of Food Technologies, 26 Maritsa Blvd., 4002 Plovdiv, Bulgaria.; Department of Physics and Biophysics, Medical University-Varna, 84 Tzar Osvoboditel Blvd., 9000 Varna, Bulgaria., Eftimov T; Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sankt Peterburg Blvd., 4002 Plovdiv, Bulgaria.; Centre de Recherche en Photonique, Université du Québec en Outaouais, 101 rue Saint-Jean-Bosco, Gatineau, QC J8Y 3G5, Canada.
Jazyk:	angličtina
Zdroj:	Foods (Basel, Switzerland) [Foods] 2024 Apr 26; Vol. 13 (9). Date of Electronic Publication: 2024 Apr 26.
DOI:	10.3390/foods13091329
Abstrakt:	An experimental study is presented on the possibility of using the fluorescence from organic dyes as a broadband light source together with a monochromator for applications in excitation-emission matrix (EEM) fluorescence spectroscopy. A high-power single-chip light-emitting diode (LED) was chosen as an excitation source with a central output wavelength at 365 nm to excite a fluorescent solution of Coumarin 1 dye dissolved in ethanol. Two excitation configurations were investigated: direct excitation from the LED and excitation through an optical-fiber-coupled LED. A Czerny-Turner monochromator with a diffraction grating was used for the spectral tuning of the fluorescence. A simple method was investigated for increasing the efficiency of the excitation as well as the fluorescence signal collection by using a diffuse reflector composed of barium sulfate (BaSO 4 ) and polyvinyl alcohol (PVA). As research objects, extra-virgin olive oil (EVOO), Coumarin 6 dye, and Perylene, a polycyclic aromatic hydrocarbon (PAH), were used. The results showed that the light-emitting-diode-induced fluorescence was sufficient to cover the losses on the optical path to the monochromator output, where a detectable signal could be obtained. The obtained results reveal the practical possibility of applying the fluorescence from dyes as a light source for food system analysis by EEM fluorescence spectroscopy.
Databáze:	MEDLINE
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