| Autor: |
Hajri L; Faculty of Sciences of Bizerte, Laboratory of Integrative Physiology, University of Carthage, Jarzouna, Bizerte 7021, Tunisia., Lewińska A; Institute of Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland., Rzeszutek I; Institute of Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland., Oklejewicz B; Institute of Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland., Wojnarowska-Nowak R; Center for Microelectronics and Nanotechnology, Institute of Materials Engineering, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland., Krogul-Sobczak A; Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland., Szpyrka E; Institute of Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland., Aires A; CITAB-Centre for the Research and Technology of Agro Environment and Biological Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal., Ghodbane S; Faculty of Sciences of Bizerte, Laboratory of Integrative Physiology, University of Carthage, Jarzouna, Bizerte 7021, Tunisia., Ammari M; Faculty of Sciences of Bizerte, Laboratory of Integrative Physiology, University of Carthage, Jarzouna, Bizerte 7021, Tunisia.; Higher Institute of Applied Biological Sciences of Tunis, University of Tunis El Manar, Tunis 1068, Tunisia., Wnuk M; Institute of Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland. |
| Abstrakt: |
Plant-derived polyphenols are bioactive compounds with potential health-promoting properties including antioxidant, anti-inflammatory, and anticancer activity. However, their beneficial effects and biomedical applications may be limited due to their low bioavailability. In the present study, we have considered a microencapsulation-based drug delivery system to investigate the anticancer effects of polyphenol-rich (apigenin, caffeic acid, and luteolin) fractions, extracted from a cereal crop pearl millet ( Pennisetum glaucum ), using three phenotypically different cellular models of breast cancer in vitro, namely triple negative HCC1806, ER-positive HCC1428, and HER2-positive AU565 cells. Encapsulated polyphenolic extract induced apoptotic cell death in breast cancer cells with different receptor status, whereas it was ineffective against non-tumorigenic MCF10F cells. Encapsulated polyphenolic extract was also found to be cytotoxic against drug-resistant doxorubicin-induced senescent breast cancer cells that were accompanied by increased levels of apoptotic and necrotic markers, cell cycle inhibitor p21 and proinflammatory cytokine IL8. Furthermore, diverse responses to the stimulation with encapsulated polyphenolic extract in senescent breast cancer cells were observed, as in the encapsulated polyphenolic extract-treated non-proliferating AU565 cells, the autophagic pathway, here cytotoxic autophagy, was also induced, as judged by elevated levels of beclin-1 and LC3b. We show for the first time the anti-breast cancer activity of encapsulated polyphenolic extract of pearl millet and postulate that microencapsulation may be a useful approach for potentiating the anticancer effects of phytochemicals with limited bioavailability. |
