Multidrug-resistant Escherichia coli strains isolated from swine manure biofertilizer in Brazil.

Autor: Oliveira-Silva M; Universidade de Ribeirão Preto (UNAERP), Avenida Costábile Romano, N° 2201. Bloco J, Lab. 1, Ribeirânia., Ribeirão Preto, SP, CEP 14096-900, Brazil., Goulart RS; Universidade de Ribeirão Preto (UNAERP), Avenida Costábile Romano, N° 2201. Bloco J, Lab. 1, Ribeirânia., Ribeirão Preto, SP, CEP 14096-900, Brazil., Moraes MA; Universidade de Ribeirão Preto (UNAERP), Avenida Costábile Romano, N° 2201. Bloco J, Lab. 1, Ribeirânia., Ribeirão Preto, SP, CEP 14096-900, Brazil., Nakamura-Silva R; Universidade de Ribeirão Preto (UNAERP), Avenida Costábile Romano, N° 2201. Bloco J, Lab. 1, Ribeirânia., Ribeirão Preto, SP, CEP 14096-900, Brazil., Fujimoto RY; Empresa Brasileira de Pesquisa Agropecuária-EMBRAPA, Unidade Tabuleiros Costeios, Av. Beira Mar, 3250 Jardins, Aracaju, Sergipe, CEP 49025040, Brazil., Sousa RC; Empresa Brasileira de Pesquisa Agropecuária-EMBRAPA, Unidade Tabuleiros Costeios, Av. Beira Mar, 3250 Jardins, Aracaju, Sergipe, CEP 49025040, Brazil., Kobayashi RKT; Laboratório de Bacteriologia Básica E Aplicada, Departamento de Microbiologia, Universidade Estadual de Londrina, Rod. Celso Garcia Cid PR 445 Km 380, Londrina, PR, CEP 86057-970, Brazil., Medeiros LP; Laboratório de Bacteriologia Básica E Aplicada, Departamento de Microbiologia, Universidade Estadual de Londrina, Rod. Celso Garcia Cid PR 445 Km 380, Londrina, PR, CEP 86057-970, Brazil., Nakazato G; Laboratório de Bacteriologia Básica E Aplicada, Departamento de Microbiologia, Universidade Estadual de Londrina, Rod. Celso Garcia Cid PR 445 Km 380, Londrina, PR, CEP 86057-970, Brazil., Pitondo-Silva A; Universidade de Ribeirão Preto (UNAERP), Avenida Costábile Romano, N° 2201. Bloco J, Lab. 1, Ribeirânia., Ribeirão Preto, SP, CEP 14096-900, Brazil. andre@pitondo.com.br.
Jazyk: angličtina
Zdroj: Environmental monitoring and assessment [Environ Monit Assess] 2024 May 10; Vol. 196 (6), pp. 534. Date of Electronic Publication: 2024 May 10.
DOI: 10.1007/s10661-024-12658-3
Abstrakt: Escherichia coli is one of the key bacteria responsible for a variety of diseases in humans and livestock-associated infections around the globe. It is the leading cause of mortality in neonatal and weaned piglets in pig husbandry, causing diarrhea and significant harm to the industry. Furthermore, the frequent and intensive use of antimicrobials for the prevention of diseases, particularly gastrointestinal diseases, may promote the selection of multidrug-resistant (MDR) strains. These resistant genotypes can be transmitted through the excrement of animals, including swine. It is common practice to use porcine manure processed by biodigesters as fertilizer. This study aimed to examine the antimicrobial susceptibility, the presence of virulence genes frequently associated with pathotypes of intestinal pathogenic E. coli (InPEC), and antimicrobial resistance genes (ARGs) of 28 E. coli isolates collected from swine manure fertilizers. In addition, the enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) technique was used to investigate the genetic relationship among the strains. Using disk diffusion, the antimicrobial susceptibility profiles of the strains were determined. Using polymerase chain reaction (PCR), 14 distinct virulence genes associated with the most prevalent diarrhea and intestinal pathogenic E. coli (DEC/InPEC) and five ARGs were analyzed. All isolates tested positive for multidrug resistance. There was no detection of any of the 14 virulence genes associated with InPECs, indicating the presence of an avirulent commensal microbiota. Molecular classification by ERIC-PCR revealed that the majority of isolates (27 isolates) coalesced into a larger cluster with a genetic similarity of 47.7%; only one strain did not cluster in this cluster, indicating a high level of genetic diversity among the analyzed isolates. Thus, it is of the utmost importance to conduct epidemiological surveillance of animal breeding facilities in order to determine their microbiota and formulate plans to reduce the use of antimicrobials and improve animal welfare.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
Externí odkaz: