Molecular characterization of emerging Echovirus 11 (E11) shed light on the recombinant origin of a variant associated with severe hepatitis in neonates.

Autor: Piralla A; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Giardina F; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., Ferrari G; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Gaiarsa S; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Romano G; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Pellegrinelli L; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy., Galli C; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy., Seiti A; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy., Binda S; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy., Pitrolo AMG; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy., Genoni A; Laboratory of Medical Microbiology and Virology, Department of Medicine and Technical Innovation, University of Insubria, Varese, Italy.; Laboratory of Medical Microbiology and Virology, University Hospital of Varese, Varese, Italy., Ferrante FD; Laboratory of Medical Microbiology and Virology, University Hospital of Varese, Varese, Italy., Novazzi F; Laboratory of Medical Microbiology and Virology, Department of Medicine and Technical Innovation, University of Insubria, Varese, Italy.; Laboratory of Medical Microbiology and Virology, University Hospital of Varese, Varese, Italy., Mancini N; Laboratory of Medical Microbiology and Virology, Department of Medicine and Technical Innovation, University of Insubria, Varese, Italy.; Laboratory of Medical Microbiology and Virology, University Hospital of Varese, Varese, Italy., Rovida F; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy., Pariani E; Department of Biomedical Sciences for Health, University of Milan, Milan, Italy., Baldanti F; Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2024 May; Vol. 96 (5), pp. e29658.
DOI: 10.1002/jmv.29658
Abstrakt: Echovirus 11 (E11) has gained attention owing to its association with severe neonatal infections. Due to the limited data available, the World Health Organization (WHO) considers public health risk to the general population to be low. The present study investigated the genetic variation and molecular evolution of E11 genomes collected from May to December 2023. Whole genome sequencing (WGS) was performed for 16 E11 strains. Phylogenetic analysis on WG showed how all Italian strains belonged to genogroup D5, similarly to other E11 strains recently reported in France and Germany all together aggregated into separate clusters. A cluster-specific recombination pattern was also identified using phylogenetic analysis of different genome regions. Echovirus 6 was identified as the major recombinant virus in 3C pro and 3D pol regions. The molecular clock analysis revealed that the recombination event probably occurred in June 2018 (95% HPD interval: Jan 2016-Jan 2020). Shannon entropy analyses, within P1 region, showed how 11 amino acids exhibited relatively high entropy. Five of them were exposed on the canyon region which is responsible for receptor binding with the neonatal Fc receptor. The present study showed the recombinant origin of a new lineage of E11 associated with severe neonatal infections.
(© 2024 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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