Inactivation of Escherichia coli , Salmonella enterica , and Listeria monocytogenes using the Contamination Sanitization Inspection and Disinfection (CSI-D) device.
| Autor: | McCoy Sanders J; Food Science Program, Chapman University, One University Drive, Orange, CA, 92866, USA., Alarcon V; Food Science Program, Chapman University, One University Drive, Orange, CA, 92866, USA., Marquis G; Food Science Program, Chapman University, One University Drive, Orange, CA, 92866, USA., Tabb A; Food Science Program, Chapman University, One University Drive, Orange, CA, 92866, USA., Van Kessel JA; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Sonnier J; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Haley BJ; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Baek I; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Qin J; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Kim M; Environmental Microbial and Food Safety Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705, USA., Vasefi F; SafetySpect Inc., Grand Forks, ND, 58202, USA., Sokolov S; SafetySpect Inc., Grand Forks, ND, 58202, USA., Hellberg RS; Food Science Program, Chapman University, One University Drive, Orange, CA, 92866, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2024 Apr 29; Vol. 10 (9), pp. e30490. Date of Electronic Publication: 2024 Apr 29 (Print Publication: 2024). |
| DOI: | 10.1016/j.heliyon.2024.e30490 |
| Abstrakt: | The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging system designed to disinfect food contact surfaces using ultraviolet-C (UVC) illumination. This study aimed to determine the optimal CSI-D parameters (i.e., UVC exposure time and intensity) for the inactivation of the following foodborne bacteria plated on non-selective media: generic Escherichia coli (indicator organism) and the pathogens enterohemorrhagic E. coli , enterotoxigenic E. coli , Salmonella enterica , and Listeria monocytogenes. Each bacterial strain was spread-plated on non-selective agar and exposed to high-intensity (10 mW/cm 2 ) or low-intensity (5 mW/cm 2 ) UVC for 1-5 s. Control plates were not exposed to UVC. The plates were incubated overnight at 37 °C and then enumerated. Three trials for each bacterial strain were conducted. Statistical analysis was carried out to determine if there were significant differences in bacterial growth between UVC intensities and exposure times. Overall, exposure to low or high intensity for 3-5 s resulted in consistent inhibition of bacterial growth, with reductions of 99.9-100 % for E. coli , 96.8-100 % for S. enterica , and 99.2-100 % for L. monocytogenes . The 1 s exposure time showed inconsistent results, with a 66.0-100 % reduction in growth depending on the intensity and bacterial strain. When the results for all strains within each species were combined, the 3-5 s exposure times showed significantly greater ( p < 0.05) growth inhibition than the 1 s exposure time. However, there were no significant differences ( p > 0.05) in growth inhibition between the high and low UVC intensities. The results of this study show that, in pure culture conditions, exposure to UVC with the CSI-D device for ≥3 s is required to achieve consistent reduction of E. coli, S. enterica, and L. monocytogenes . Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Authors FV and SS own stocks or stock options in SafetySpect Inc. FV is an inventor on US patent application US20210228757A1. The remaining authors declare no conflict of interest. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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