Susceptibility and transmissibility of SARS-CoV-2 variants in transgenic mice expressing the cat angiotensin-converting enzyme 2 (ACE-2) receptor.

Autor:	Jiménez de Oya N; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Calvo-Pinilla E; Centro de Investigación en Sanidad Animal, INIA-CSIC. Carretera Algete-El Casar de Talamanca, Km. 8,1, 28130 Valdeolmos, Madrid, Spain., Mingo-Casas P; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Escribano-Romero E; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Blázquez AB; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Esteban A; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Fernández-González R; Departamento de Reproducción Animal, INIA-CSIC. Av. Puerta de Hierro, 18, Madrid 28040, Spain., Pericuesta E; Departamento de Reproducción Animal, INIA-CSIC. Av. Puerta de Hierro, 18, Madrid 28040, Spain., Sánchez-Cordón PJ; Centro de Investigación en Sanidad Animal, INIA-CSIC. Carretera Algete-El Casar de Talamanca, Km. 8,1, 28130 Valdeolmos, Madrid, Spain., Martín-Acebes MA; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain., Gutiérrez-Adán A; Departamento de Reproducción Animal, INIA-CSIC. Av. Puerta de Hierro, 18, Madrid 28040, Spain., Saiz JC; Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC). Ctra. de La Coruña, km 7, 5, Madrid 28040, Spain.
Jazyk:	angličtina
Zdroj:	One health (Amsterdam, Netherlands) [One Health] 2024 Apr 29; Vol. 18, pp. 100744. Date of Electronic Publication: 2024 Apr 29 (Print Publication: 2024).
DOI:	10.1016/j.onehlt.2024.100744
Abstrakt:	The emergence of SARS-CoV-2 in 2019 and its rapid spread throughout the world has caused the largest pandemic of our modern era. The zoonotic origin of this pathogen highlights the importance of the One Health concept and the need for a coordinated response to this kind of threats. Since its emergence, the virus has caused >7 million deaths worldwide. However, the animal source for human outbreaks remains unknown. The ability of the virus to jump between hosts is facilitated by the presence of the virus receptor, the highly conserved angiotensin-converting enzyme 2 (ACE2), found in various mammals. Positivity for SARS-CoV-2 has been reported in various species, including domestic animals and livestock, but their potential role in bridging viral transmission to humans is still unknown. Additionally, the virus has evolved over the pandemic, resulting in variants with different impacts on human health. Therefore, suitable animal models are crucial to evaluate the susceptibility of different mammalian species to this pathogen and the adaptability of different variants. In this work, we established a transgenic mouse model that expresses the feline ACE2 protein receptor (cACE2) under the human cytokeratin 18 (K18) gene promoter's control, enabling high expression in epithelial cells, which the virus targets. Using this model, we assessed the susceptibility, pathogenicity, and transmission of SARS-CoV-2 variants. Our results show that the sole expression of the cACE2 receptor in these mice makes them susceptible to SARS-CoV-2 variants from the initial pandemic wave but does not enhance susceptibility to omicron variants. Furthermore, we demonstrated efficient contact transmission of SARS-CoV-2 between transgenic mice that express either the feline or the human ACE2 receptor. Competing Interests: The authors report there are no competing interests to declare. (© 2024 The Authors. Published by Elsevier B.V.)
Databáze:	MEDLINE
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