Osteogenic potential of Frondoside A in human periodontal ligament cells: an RNA-Seq analysis.

Autor:	Kim Y; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Kang SW; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Ye JR; Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea., Kim SE; Interdisciplinary Program of Biomedical Engineering, Graduate School, Chonnam National University, Gwangju, Korea., Chae YK; Department of Pediatric Dentistry, Kyung Hee University, College of Dentistry, Kyung Hee University Medical Center, Seoul, Korea., Nam OH; Department of Pediatric Dentistry, Kyung Hee University, College of Dentistry, Kyung Hee University Medical Center, Seoul, Korea.; Department of Pediatric Dentistry, Kyung Hee University School of Dentistry, Seoul, Korea. pedokhyung@gmail.com.
Jazyk:	angličtina
Zdroj:	Journal of periodontal & implant science [J Periodontal Implant Sci] 2024 Apr 17. Date of Electronic Publication: 2024 Apr 17.
DOI:	10.5051/jpis.2303840192
Abstrakt:	Purpose: The aim of this study was to evaluate the effects of Frondoside A (FA) on the osteogenic differentiation of human periodontal ligament (PDL) cells. Methods: Human PDL cells were cultured in osteogenic medium and treated with FA at concentrations of 0, 0.05, and 0.2 µM for 14 days. The expression levels of genes associated with osteogenic differentiation were assessed using quantitative real-time polymerase chain reaction analysis. Subsequently, RNA sequencing was performed to identify enriched gene sets following FA treatment. Alkaline phosphatase (ALP) activity was measured to confirm the osteogenic potential of FA. Results: Treatment with 0.2 µM FA significantly increased the expression levels of runt-related transcription factor 2 ( RUNX2 ), ALP , and osteocalcin ( OCN ) at day 3, while also significantly elevating the expression of dentin sialophosphoprotein ( DSPP ), RUNX2 , ALP , OCN , and osterix ( OSX ) at day 14 ( P <0.017). Hallmark gene sets enriched during FA treatment were associated with the KRAS (normalized enrichment score [NES]=2.02, Q =0.000), interferon alpha (IFN-α) (NES=1.88, Q =0.001), IFN-γ (NES=1.85, Q <0.001), hypoxia (NES=1.79, Q =0.001), and p53 (NES=1.77, Q =0.001) signaling pathways. Additionally, treatment with 0.2 µM FA significantly intensified ALP staining at day 14 ( P <0.05). Conclusions: Within the limitations of this study, FA treatment influenced periodontal regeneration by promoting the osteogenic differentiation of human PDL cells. Competing Interests: No potential conflict of interest relevant to this article was reported. (Copyright © 2024. Korean Academy of Periodontology.)
Databáze:	MEDLINE
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