Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study.

Autor: Shid-Moosavi TS; Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran., Mohammadi N; Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. najme_mohammadi64@yahoo.com.; Oral and Dental Disease Research Center, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran. najme_mohammadi64@yahoo.com., Gharamani Y; Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran., Motamedifar M; Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran., Alizadeh AA; Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk: angličtina
Zdroj: European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry [Eur Arch Paediatr Dent] 2024 Jun; Vol. 25 (3), pp. 443-450. Date of Electronic Publication: 2024 May 09.
DOI: 10.1007/s40368-024-00905-7
Abstrakt: Purpose: This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide-eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs).
Methods: The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay.
Results: The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001).
Conclusion: Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE.
(© 2024. The Author(s), under exclusive licence to European Academy of Paediatric Dentistry.)
Databáze: MEDLINE
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